Abstract
The v-myc oncogene can induce tumours in haematopoietic, mesenchymal and epithelial tissues1. The corresponding c-myc proto-oncogene can contribute to the genesis and/or the progression of an equally wide variety of tumours when activated by retroviral insertions, chromosomal translocations or gene amplification2. The c-myc gene product is a DNA-binding, nuclear phosphoprotein that is involved in the control of cell proliferation and possibly in DNA synthesis2. The replication of Simian virus 40 (SV40) is a useful model system to study eukaryotic DNA replication as the virus relies almost entirely on cellular DNA replication apparatus. The SV40-based vector, pSVEpR4, replicates poorly in the human BJAB lymphoma line and in most human cells, but replicates well in Burkitt lymphoma lines, which have fused immunoglobulin and c-myc genes, resulting in high c-myc expression. Cotransfection of the BJAB cells with a c-myc-expressing construct (pI4-P6) increased the replication of pSVEpR4 tenfold. Our findings indicate that overexpression of the c-myc gene product allows the replication of SV40 in human lymphoma cells, suggesting that c-myc is involved in the control of replication.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
1. Moscovici, C. & Purchio, A. F. Adv. Viral Oncol 1, 83-106 (1982). 2. Bishop, J. M. Science 235, 305-311 (1987). 3. Klein, G., Giovanella, B., Westman, A., Stehlin, J. S. & Mumford, D. Intervirology 5, 319-334 (1975). 4. Hammarskjold, M.-L., Wang, S.-C. & Klein, G. Gene 43, 41-51 (1986). 5. McCutchan, J. H. & Pagano, J. /. natn. Cancer Inst. 41, 351-357 (1968). 6. Hirt, B. J. molec. Biol. 26, 365-369 (1967). 7. Pulvertaft, R. I. V. /. din. Path. 18, 261-273 (1965). 8. Klein, E. et al. Cancer Res. 28, 1300-1310 (1968). 9. Van Santen, V., Cheung, A. & Kieff, E. Proc. natn. Acad. Sci. U.S.A. 78, 1930-1934 (1981). 10. Klein, G. Cell 32, 311-315 (1983). 11. Menezes, J., Leibold, W., Klein, G. & Clements, G. B. Biomedicine 22, 276-284 (1975). 12. Klein, G. et al. Int. J. Cancer 18, 639-652 (1976). 13. Wennborg, A. et al. Int. J. Cancer 40, 202-206 (1987). 14. Clements, G. B., Klein, G. & Povey, S. Int. J. Cancer 16, 125-133 (1975). 15. Fresen, K. O. & zur Hausen, H. Int. J. Cancer 17, 161-166 (1976). 16. Gerhard, R. D., Guggenheimer, R. A. & Gluzman, Y. /. Virol. 61, 851-857 (1987). 17. Gerhard, R. D. & Gluzman, Y. Molec. cell. Biol. 5, 3231-3240 (1985). 18. Gluzman, Y., Sambrook, J. & Frisque, J. Proc. natn. Acad. Sci. U.S.A. 77,3398-3902 (1980). 19. Kaddurah-Daouk, R., Greene, J. M., Baldwin, A. S. Jr & Kingston, R. E. Genes Dev. 1, 347-357 (1987). 20. Lewis, E. D. & Manley, J. L. Nature 317, 172-175 (1985). 21. Lebkowski, J. S., Clancy, S. & Calos, M. R Nature 317, 169-171 (1985). 22. Land, H., Parada, L. F. & Weinberg, R. A. Science 234, 467-470 (1983). 23. Ruley, H. E. Nature 304, 602-606 (1983). 24. Geier, G. E. & Modrich, P. /. biol. Chem. 254, 1408-1413 (1979). 25. Southern, E. M. /. molec. Biol. 98, 503-517 (1975). 26. Watt, R. et al. Proc. natn. Acad. Sci. U.S.A 80, 6307-6311 (1983). 27. Winqvist, R., Saksela, K. & Alitalo, K. EMBO J. 12, 2947-2950 (1984). 28. Alitalo, K., Schwab, M., Lin, C. C., Varmus, H. & Bishop, J. M. Proc. natn. Acad. Sci. C/.& A80, 1707-1711 (1983). 29. Laemmli, U. K. Nature 227, 680-685 (1970). 30. Towbin, H. & Gordon, J. Proc. natn. Acad. Sci. U.S.A. 76, 4350-4355 (1979).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Classon, M., Henriksson, M., Sümegi, J. et al. Elevated c-myc expression facilitates the replication of SV40 DNA in human lymphoma cells . Nature 330, 272–274 (1987). https://doi.org/10.1038/330272a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/330272a0
This article is cited by
-
MYC: a multipurpose oncogene with prognostic and therapeutic implications in blood malignancies
Journal of Hematology & Oncology (2021)
-
Simian virus 40 and its association with human lymphomas
Current Oncology Reports (2003)
-
c-Myc initiates illegitimate replication of the ribonucleotide reductase R2 gene
Oncogene (2002)
-
erratum: Elevated c-myc expression facilitates the replication of SV40 DNA in human lymphoma cells
Nature (2001)
-
Chromosomal and Extrachromosomal Instability of the cyclin D2 Gene is Induced by Myc Overexpression
Neoplasia (1999)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
