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Amino-acid sequence of the catalytic subunit of the (Na+ + K+)ATPase deduced from a complementary DNA

Nature volume 316, pages 691–695 (1985)Cite this article

Abstract

We have isolated and characterized a complementary DNA for the catalytic subunit of the sheep kidney sodium / potassium-dependent ATPase. The 1,016-amino-acid protein seems to have eight transmembrane domains. The apparent ouabain binding site is located at the extracellular junction of two transmembrane domains and is linked to the phosphorylation site by a 60-amino-acid conserved sequence that may be a major channel for energy transduction.

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Authors and Affiliations

  1. Department of Microbiology and Molecular Genetics, University of Cincinnati College of Medicine, 231 Bethesda Avenue, Cincinnati, Ohio, 45267, USA

    Gary E. Shull & Jerry B. Lingrel

  2. Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, 231 Bethesda Avenue, Cincinnati, Ohio, 45267, USA

    Arnold Schwartz

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  1. Gary E. Shull
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  2. Arnold Schwartz
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  3. Jerry B. Lingrel
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Shull, G., Schwartz, A. & Lingrel, J. Amino-acid sequence of the catalytic subunit of the (Na+ + K+)ATPase deduced from a complementary DNA. Nature 316, 691–695 (1985). https://doi.org/10.1038/316691a0

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