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Expression of human immune interferon cDNA in E. coli and monkey cells

Abstract

A cDNA sequence coding for human immune interferon (IFN-γ) has been identified in a cDNA library prepared from gel-fractionated IFN-γ mRNA. The DNA sequence codes for a polypeptide of 166 amino acids, 20 of which could constitute a signal peptide. The polypeptide produced through expression of this DNA sequence in Escherichia coli or cultured monkey cells had properties characteristic of authentic human IFN-γ.

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Gray, P., Leung, D., Pennica, D. et al. Expression of human immune interferon cDNA in E. coli and monkey cells. Nature 295, 503–508 (1982). https://doi.org/10.1038/295503a0

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