Abstract
TRANSPLANTATION antigen-like tumour-associated surface membrane antigens (TATA) have now been prepared in solubilised or semi-solubilised form1–3. The methods of preparation are, however, complicated and yields are usually poor. Moreover, protection experiments with solubilised tumour antigens have rarely been successful and have given very limited protection1,2,4,5. On the other hand, all manner of devitalised cells protect in immunisation experiments against living tumour cells6; and it is therefore possible that interference with cell membrane integrity during solubilisation destroys tertiary and quaternary structures important for the immunogenicity of membrane antigens6,7. We describe here a simple and gentle procedure for preparing in near-quantitative yield (nucleated) tumour cell ‘ghosts’ with retention of protective immunogenicity. Freeze drying of ‘ghost’ suspensions provided a stable material which retained its protective activity for 2.5 yr when stored dry at 4 °C.
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References
Meltzer, M. S., Leonard, E. J., Rapp, H. J., and Borsos, T., J. natn. Cancer Inst., 47, 703 (1971).
Baldwin, R. W., and Glaves, D., Clin. exp. Immun., 11, 51 (1972).
Kedar, E., Aronov, A., Sulitzenau, D., Israel J. med. Sci., 9, 266 (1973).
Law, L. W., and Appella, E. Nature, 243, 83 (1973).
Holmes, E. C., Kahan, B. D., and Morton, D. C., Cancer, 25, 373 (1970).
Augustin, R., Nature, 224, 295 (1969).
Wolf, A., and Avis, P. J. G., Transplantation, 9, 18 (1970).
Ponder, E., J. exp. Biol., 18, 257 (1942); in The Cell, 2 (edit. by Brachet, J., and Mirsky, A. E.), (Academic, New York, 1961).
Dodge, J. T., Mitchell, C., and Hanahan, D. J., Archs Biochem. Biophys., 100, 119 (1962).
Wallach, D. F. H., and Ullrey, D., Biochim. biophys. Acta, 64, 526 (1964).
Emmelot, P., and Bos, C. J., Biochim. biophys. Acta, 59, 375 (1966).
Bos, C. J., and Emmelot, P., Int. J. Cancer, 4, 705 (1969).
Fitzpatrick, M. E., et al., Immunology, 12, 1 (1967).
West, C. R., and Augustin, R., in Cytology Automation (edit. by Evans, D. M. D.), 121 (Proc. second Tenovus Symp., Livingstone, 1970).
Augustin, R., West, C. R., Sparshott, S. M., and Chandradasa, K. D., in Immunity and Tolerance in Oncogenesis, 2 (edit. by Severi, L.), 931 (Perrugia University Press, Italy, 1970).
Winn, H. J., J. Immun., 86, 228 (1961).
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DAVIES, I., NICKLIN, M. & AUGUSTIN, R. Protective tumour cell ghosts with intact membrane markers. Nature 256, 49–50 (1975). https://doi.org/10.1038/256049a0
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DOI: https://doi.org/10.1038/256049a0
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