Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Intragenic regulation of the synthesis of ΦX174 gene A proteins

Nature volume 249, pages 345–348 (1974)Cite this article

Abstract

ONLY one phage gene, A, has been shown to be necessary for the replication of ΦX174 double-stranded replicative form (RF) DNA1–3. The host genes required have yet to be fully determined although one host mutation, rep−3, prevents progeny RF synthesis4. Dissection of the characteristics of gene A has shown that it is (a) cis acting5; (b) necessary for the detection of a cis-related viral strand specific discontinuity in the parental RF6,7; (c) the region of the genome which contains the origin of DNA replication8, and (d) a gene from which two proteins, A and A′ (molecular weights 35,000 and 60-65,000, respectively) are synthesised9,10.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Tessman, E. S., J. molec. Biol., 17, 218 (1966).

    Article  CAS  Google Scholar 

  2. Lindqvist, B. H., and Sinsheimer, R. L., J. molec. Biol., 30, 69 (1967).

    Article  CAS  Google Scholar 

  3. Iwaya, M., and Denhardt, D. T., J. molec. Biol., 57, 159 (1971).

    Article  CAS  Google Scholar 

  4. Denhardt, D. T., Dressler, D. H., and Hathaway, A., Proc. natn. Acad. Sci. U.S.A., 57, 803 (1967).

    Article  ADS  Google Scholar 

  5. Tessman, E. S., Virology, 25, 303 (1965).

    Article  CAS  Google Scholar 

  6. Francke, B., and Ray, D. S., J. molec. Biol., 61, 565 (1971).

    Article  CAS  Google Scholar 

  7. Francke, B., and Ray, D. S., Proc. natn. Acad. Sci. U.S.A., 69, 475 (1972).

    Article  ADS  CAS  Google Scholar 

  8. Baas, D. D., and Jansz, H. S., J. molec. Biol., 63, 569 (1972).

    Article  CAS  Google Scholar 

  9. Linney, E. A., Hayashi, M. N., and Hayashi, M., Virology, 50, 381 (1972).

    Article  CAS  Google Scholar 

  10. Linney, E., and Hayashi, M., Nature new Biol., 245, 6 (1973).

    Article  CAS  Google Scholar 

  11. Gelfand, D. H., and Hayashi, M., J. molec. Biol., 44, 501 (1969).

    Article  CAS  Google Scholar 

  12. Burgess, A. B., and Denhardt, D. T., J. molec. Biol., 44, 377 (1969).

    Article  CAS  Google Scholar 

  13. Godson, G. N., J. molec. Biol., 57, 541 (1971).

    Article  CAS  Google Scholar 

  14. Benbow, R. M., Mayol, R. F., Picchi, J. C., and Sinsheimer, R. L., J. Virol., 10, 99 (1972).

    CAS  PubMed  PubMed Central  Google Scholar 

  15. Van der Mei, D., Zandberg, J., and Jansz, H. S., Biochim. biophys. Acta, 287, 312 (1972).

    Article  CAS  Google Scholar 

  16. Sinsheimer, R. L., Hutchison, C. A., and Lindqvist, B., in Molecular biology of viruses, 175 (Academic. Press, New York 1967).

    Book  Google Scholar 

  17. Hayashi, Y., and Hayashi, M., Cold Spring Harbor Symp. quant. Biol., 35, 171 (1970).

    Article  CAS  Google Scholar 

  18. Vanderbilt, A. S., Borrás, M. T., Germeraad, S., Tessman, I., and Tessman, E. S., Virology, 50, 171 (1972).

    Article  CAS  Google Scholar 

  19. Puga, A., Borrás, M. T., Tessman, E. S., and Tessman, I., Proc. natn. Acad. Sci. U.S.A., 70, 2171 (1973).

    Article  ADS  CAS  Google Scholar 

  20. Silverstein, S., and Billew, D., Biochim. biophys. Acta, 241, 383 (1971).

    Article  Google Scholar 

  21. Brutlag, D., Schekman, R. W., and Kornberg, A., Proc. natn. Acad. Sci. U.S.A., 68, 2826 (1971).

    Article  ADS  CAS  Google Scholar 

  22. Van der Mei, D., Brons, J. Th., and Jaisz, H. S., Biochim. biophys. Acta, 262, 463 (1972).

    Article  CAS  Google Scholar 

  23. Suruda, A. J., and Poljak, R. J., Virology, 46, 164 (1971).

    Article  CAS  Google Scholar 

  24. Zuccarelli, A., Benbow, R. M., and Sinsheimer, R. L., Proc. natn. Acad. Sci. U.S.A., 69, 1905 (1972).

    Article  ADS  CAS  Google Scholar 

  25. Hayashi, M., Hayashi, M. N., and Spiegelman, S., Proc. natn. Acad. Sci. U.S.A., 50, 664 (1963).

    Article  ADS  CAS  Google Scholar 

  26. Vanderbilt, A. S., and Tessman, I., Nature, 228, 54 (1971).

    Article  ADS  Google Scholar 

  27. Tessman, I., Kumar, S., and Tessman, E. S., Science, 158, 267 (1967).

    Article  ADS  CAS  Google Scholar 

  28. Vanderbilt, A. S., Borrás, M., and Tessman, E. S., Virology, 43, 352 (1971).

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. ELWOOD LINNEY

    Present address: Department of Microbiology, State University of New York, Stony Brook, New York, 11790

Authors and Affiliations

  1. Department of Biology, University of California, San Diego, La Jolla, California, 92037

    ELWOOD LINNEY & MASAKI HAYASHI

Authors
  1. ELWOOD LINNEY
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. MASAKI HAYASHI
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

LINNEY, E., HAYASHI, M. Intragenic regulation of the synthesis of ΦX174 gene A proteins. Nature 249, 345–348 (1974). https://doi.org/10.1038/249345a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/249345a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing