Abstract
ULTRA-VIOLET irradiation produces injuries in bacteria which can be repaired in certain strains either by exposure to light (photoreactivation)1 or by incubation in the dark2,3. Aphotoreactivating enzyme extracted from yeast reconverts thymine dimers present in ultra-violet-irradiated deoxyribonucleic acid (DNA) to thymine4. An enzymatic mechanism has also been proposed for the dark reactivation of ultra-violet-irradiated bacteriophage in E. coli B (refs. 5–7). This process was not inhibited by thymine deprivation8. In bacteria, dark reactivation can be demonstrated by recovery of the ability to synthesize acid-insoluble DNA following ultra-violet irradiation9. It, therefore, was of interest to see if this reactivation could also proceed under conditions of thymine deprivation.
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SHUSTER, R. Dark Repair of Ultra-violet Injury in E. coli during Deprivation of Thymine. Nature 202, 614–615 (1964). https://doi.org/10.1038/202614a0
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DOI: https://doi.org/10.1038/202614a0
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