Abstract
IN genetic analysis based on mitotic segregation1–4 an important step is the recovery of haploid segregants from heterozygous diploids. This has been done until recently by laborious or complicated techniques4,5. I am indebted to Dr. G. Morpurgo, of the Istituto Superiore di Sanità, Rome, for informing me and permitting me to make use of his discovery that treatment with para-fluoro-phenylalanine of diploids of Aspergillus nidulans leads to wholesale segregation (this discovery was made known in the privately circulated Aspergillus News Letter, p. 10, No. 2, Spring 1961). In an extensive work expanding the formal genetical analysis—via mitotic segregation—of the fungus A. niger 6, I have applied this treatment to diploids of this asexual species.
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LHOAS, P. Mitotic Haploidization by Treatment of Aspergillus niger Diploids with para-Fluorophenylalanine. Nature 190, 744 (1961). https://doi.org/10.1038/190744a0
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DOI: https://doi.org/10.1038/190744a0
