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Determination of Yeast Carbohydrates with the Anthrone Reagent

Abstract

A PAPER on this subject by Fales1 has recently appeared; almost simultaneously, we described a more extensive fractionation of yeast carbohydrates, using also the anthrone reagent for colorimetric determinations of total cell carbohydrate as well as of the individual fractions2. Considerable interest has been shown in this technique, as judged by the volume of correspondence we have received. As the original procedure was adapted to the small amounts of yeast used in manometric experiments on fermentation3 and requires considerable manipulative skill and time, we wish to put on record the following adaptation which can be used when larger quantities of yeast are available for analysis. The dilutions of the various fractions are designed to give about the same optical density in the anthrone reaction as a standard solution containing 100 µgm. glucose/ml., when D.C.L. baker's yeast is used; other yeasts may require some adjustment in this respect.

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References

  1. Fales, F. W., J. Biol. Chem., 193, 113 (1951).

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  2. Trevelyan, W. E., and Harrison, J. S., Biochem. J., 50, 298 (1951).

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  3. Trevelyan, W. E., Gammon, J. N., Wiggins, E. H., and Harrison, J. S., Biochem. J., 50, 303 (1951).

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TREVELYAN, W., FORREST, R. & HARRISON, J. Determination of Yeast Carbohydrates with the Anthrone Reagent. Nature 170, 626–627 (1952). https://doi.org/10.1038/170626a0

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