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  • Original Paper
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MTG8 proto-oncoprotein interacts with the regulatory subunit of type II cyclic AMP-dependent protein kinase in lymphocytes

Oncogene volume 20, pages 6225–6232 (2001)Cite this article

Abstract

AML1-MTG8 chimeric oncogene is generated in acute myelogenous leukemia with t(8;21), and seems to be responsible for the pathogenesis of the disease. However, the role of MTG8 is ambiguous. Here we found that MTG8 interacted with the regulatory subunit of type II cyclic AMP-dependent protein kinase (PKA RIIα). The binding site of MTG8 was NHR3 domain, and that of RIIα was the N-terminus for interacting with PKA anchoring proteins (AKAPs). NHR3 contains a putative α-amphipathic helix which is characteristic in binding of AKAPs with RII. Indirect immunofluorescence microscopy showed that MTG8 and RIIα were overlapped at the centrosome-Golgi area in lymphocytes. These findings suggest that MTG8 may function as an AKAP at the centrosome-Golgi area in lymphocytes.

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Acknowledgements

We would like to express our thanks to Professor Kinji Inoue (Laboratory of Cell Biology, Department of Regulation Biology, Faculty of Science, Saitama University, Japan) for advice on confocal microscopy. This work was supported in part by Grants-in-Aid for Science from the Ministry of Education, Science, Sports, and Culture of Japan, Grant of Clinical Research Foundation (2000) as well as Cutting Edge Fundamental Research from Saitama Prefecture; and grants from the Organization for Pharmaceutical Safety and Research (OPSR), and from the Kawano Memorial Foundation for Promotion of Pediatrics, Japan.

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Authors and Affiliations

  1. Saitama Cancer Center Research Institute, Saitama, 362-0806, Japan

    Tomofusa Fukuyama, Eisaburo Sueoka, Kiwamu Akagi & Tomoko Kozu

  2. Cancer Center, Kyushu University Hospital, Fukuoka, 812-8582, Japan

    Tomofusa Fukuyama, Yasuhiro Sugio & Teruhisa Otsuka

  3. Department of Medicine and Biosystemic Science, Faculty of Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, 812-8582, Japan

    Yoshiyuki Niho

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  1. Tomofusa Fukuyama
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Correspondence to Tomoko Kozu.

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Fukuyama, T., Sueoka, E., Sugio, Y. et al. MTG8 proto-oncoprotein interacts with the regulatory subunit of type II cyclic AMP-dependent protein kinase in lymphocytes. Oncogene 20, 6225–6232 (2001). https://doi.org/10.1038/sj.onc.1204794

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