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Trends in large-scale mouse mutagenesis: from genetics to functional genomics

Nature Reviews Genetics volume 9, pages 803–810 (2008)Cite this article

Abstract

The primary goal of mouse mutagenesis programmes is to develop a fundamental research infrastructure for mammalian functional genomics and to produce human disease models. Many large-scale programmes have been ongoing since 1997; these culminated in the International Knockout Mouse Consortium (IKMC) in 2007 with the aim to establish knockout and conditional mouse strains for all mouse genes. This article traces the origins and rationale of these large-scale mouse mutagenesis programmes.

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Figure 1: Mating scheme of ENU-mutagenesis protocols in the mouse.
Figure 2: Knockout and trapping mutagenesis and related infrastructures.
Figure 3: Outline of ENU-based gene-driven mutagenesis.

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Author information

Authors and Affiliations

  1. Yoichi Gondo is a member of the Mutagenesis and Genomics Team, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, 305-0074, Ibaraki, Japan

    Yoichi Gondo

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  1. Yoichi Gondo
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Supplementary information

Supplementary information S1 (box)

Key mutation numbers and rates in ENU mutagenesis. (PDF 213 kb)

Related links

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FURTHER INFORMATION

AMMRA, Asian Mouse Mutagenesis and Resource Association

APF, Australian Phenomics Facility

Baylor College of Medicine ENU mouse mutagenesis

CARD, Center for Animal Resources and Development

CASIMIR, Coordination and Sustainability of International Mouse Informatics Resources

CMHD, Center for Modeling Human Disease

DBCLS, Database Center for Life Sciences

EMMA, European Mouse Mutant Archive

EMPReSS, European Mouse Phenotyping Resource of Standardized Screen (SOP database)

EuCOMM, European Conditional Mouse Mutagenesis

EUMODIC, The European Mouse Disease Clinic

Eumorphia, European Phenotyping Consortium 2002–2006

EURExpress II, gene expression atlas by RNA in situ

EuroPhenome, Europhenome Mouse Phenotyping Resource

Experimental Animal Division, RIKEN BioResource Center

FIMRe, Federation of International Mouse Resources

GMC, German Mouse Clinic

Helmholtz Zentrum Munchen German Research Center ENU mouse mutagenesis

IGTC, International Gene Trap Consortium

IMSR, International Mouse Strain Resource

INGENOtyping for ENU gene-driven mutagenesis

JAX Mice, The Jackson Laboratory

JAX Mouse Heart, Lung, Blood and Sleep Disorders Center

JAX Mouse Phenome Database

JAX Neuroscience Mutagenesis Facility

JMSR, Japan Mouse/Rat Strain Resource Database

KOMP, Knockout Mouse Project

McLaughlin Research Institue ENU mouse mutagenesis

Medical Research Council, archive for ENU gene-driven mutagenesis

Medical Research Council ENU mouse mutagenesis

MGI, Mouse Genome Informatics

MMRRC, Mutant Mouse Regional Resource Centers

NorCOMM, North American Conditional Mouse Mutagenesis

Northwestern University ENU mouse mutagenesis

Oak Ridge National Laboratory Mutant Mouse Database

PBmice, PiggyBac Mutagenesis Information Center

RIKEN ENU mouse mutagenesis

RIKEN Mutant Mouse Library, ENU-based gene-driven mutagenesis

TCP, Toronto Centre for Phenogenomics

Tennessee Mouse Genome Consortium ENU mouse mutagenesis

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Gondo, Y. Trends in large-scale mouse mutagenesis: from genetics to functional genomics. Nat Rev Genet 9, 803–810 (2008). https://doi.org/10.1038/nrg2431

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