Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Advertising Feature: Application Note
  • Published:

Multiphoton microscopy

Abstract

The next evolution in multiphoton microscopy will further enhance the ability to observe complex and dynamic biological processes from deeper within living tissue with minimal invasion and photodamage.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Schematic of Nonlinear Signal Generation:
Figure 2: Representative Multiphoton Images:
Figure 3: Broadband Two-Photon Excitation:

References

  1. Kobat, D. et al. Deep tissue multiphoton microscopy using longer wavelength excitation. Opt. Express. 17, 13354–13364 (2009).

    Article  ADS  Google Scholar 

  2. Pang, S., Yeh, A. T., Wang, C. Meissner, K. E. Beyond the 1/Tp limit: two-photon excited fluorescence using pulses as short as sub-10-fs. J. Biomed. Opt. 14, 054041 (2009).

    Article  ADS  Google Scholar 

  3. Tang, S. Krasieva, T. B., Chen Z., Tempea, G., Tromberg, B. J. Effect of pulse duration on two-photon excited fluorescence and second harmonic generation in nonlinear optical microscopy. J. Biomed. Opt. 11, 020501 (2006).

    Article  ADS  Google Scholar 

  4. Larson, A. M. and Yeh, A. T. Ex-Vivo characterization of sub-10-fs pulses. Opt. Lett. 31, 1681–1683 (2006).

    Article  ADS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Larson, A. Multiphoton microscopy. Nature Photon 5, 1 (2011). https://doi.org/10.1038/nphoton.an.2010.2

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nphoton.an.2010.2

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing