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Test of synergistic interactions among deleterious mutations in bacteria

Nature volume 390, pages 395–398 (1997)Cite this article

Abstract

Identifying the forces responsible for the origin and maintenance of sexuality remains one of the greatest unsolved problems in biology1,2,3,4,5,6. The mutational deterministic hypothesis postulates that sex is an adaptation that allows deleterious mutations to be purged from the genome; it requires synergistic interactions, which means that two mutations would be more harmful together than expected from their separate effects4,5. We generated 225 genotypes of Escherichia coli carrying one, two or three successive mutations and measured their fitness relative to an unmutated competitor. The relationship between mutation number and average fitness is nearly log-linear. We also constructed 27 recombinant genotypes having pairs of mutations whose separate and combined effects on fitness were determined. Several pairs exhibit significant interactions for fitness, but they are antagonistic as often as they are synergistic. These results do not support the mutational deterministic hypothesis for the evolution of sex.

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Figure 1: Hypothetical effects of increasing numbers of deleterious mutations on fitness.
Figure 2: Observed effect of increasing the number of deleterious mutations on fitness in the bacterium, E.coli .
Figure 3: Distribution of observed minus expected fitness values for 27 different double mutants.

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Acknowledgements

We thank V. de Lorenzo, L. Forney, J. Peck and O. Schabenberger for advice and encouragement; L. Ekunwe and N. Hajela for technical support; and A. de Visser, J. Mongold, P.Moore, S.Otto, B. Rice, D. Rozen and C. Zeyl for valuable comments. This work was supported by a fellowship from the Spanish MEC to S.F.E. and a grant from the NSF to R.E.L.

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  1. Center for Microbial Ecology, Michigan State University, East Lansing, 48824, Michigan, USA

    Santiago F. Elena & Richard E. Lenski

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  1. Santiago F. Elena
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  2. Richard E. Lenski
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Correspondence to Santiago F. Elena.

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Elena, S., Lenski, R. Test of synergistic interactions among deleterious mutations in bacteria. Nature 390, 395–398 (1997). https://doi.org/10.1038/37108

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