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Unicellular C4 photosynthesis in a marine diatom

Nature volume 407, pages 996–999 (2000)Cite this article

Abstract

Nearly 50 years ago, inorganic carbon was shown to be fixed in microalgae as the C3 compound phosphoglyceric acid1. The enzyme responsible for C3 carbon fixation, ribulose-1,5-bisphosphate carboxylase (Rubisco), however, requires inorganic carbon in the form of CO2 (ref. 2), and Rubisco enzymes from diatoms have half-saturation constants for CO2 of 30–60 µM (ref. 3). As a result, diatoms growing in seawater that contains about 10 µM CO2 may be CO2 limited4. Kinetic and growth studies have shown that diatoms can avoid CO2 limitation5,6,7, but the biochemistry of the underlying mechanisms remains unknown. Here we present evidence that C4 photosynthesis supports carbon assimilation in the marine diatom Thalassiosira weissflogii, thus providing a biochemical explanation for CO2-insensitive photosynthesis in marine diatoms. If C4 photosynthesis is common among marine diatoms, it may account for a significant portion of carbon fixation and export in the ocean, and would explain the greater enrichment of 13C in diatoms compared with other classes of phytoplankton. Unicellular C4 carbon assimilation may have predated the appearance of multicellular C4 plants.

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Figure 1: Phosphoenolpyruvate carboxylase (PEPCase) activities (squares) and growth rates (+) in the marine diatom T. weissflogii.
Figure 2: Short-term 14C assimilation in the marine diatom T. weissflogii.
Figure 3: Transfer of 14C from malate to C3 compounds (PGA and sugars) in the marine diatom T. weissflogii.

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Acknowledgements

We wish to thank M. Spring for help with the enzyme analyses; S.I. Chang, C.-W. Fan; and P.D. Tortell for help with the pulse-chase experiments; I. Schaperdoth for help with the cell fractionation; and T. Lane and K. Keller for useful comments. Funding from the Center for Environmental Bioinorganic Chemistry supported by NSF and DOE.

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Authors and Affiliations

  1. Department of Environmental Sciences Rutgers University, 14 College Farm Road, New Brunswick, 08901, New Jersey, USA

    John R. Reinfelder

  2. Centre de Geochimie de la Surface, Université de Strasbourg, 1 rue Blessig, Strasbourg, 67084, France

    Anne M. L. Kraepiel

  3. Department of Geosciences, Princeton University, Princeton, 08544, New Jersey, USA

    François M. M. Morel

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  1. John R. Reinfelder
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  2. Anne M. L. Kraepiel
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  3. François M. M. Morel
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Correspondence to John R. Reinfelder.

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Reinfelder, J., Kraepiel, A. & Morel, F. Unicellular C4 photosynthesis in a marine diatom. Nature 407, 996–999 (2000). https://doi.org/10.1038/35039612

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