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Ebselen Protects Ca2+ Influx Blockage But Does Not Protect Glutamate Uptake Inhibition Caused By Hg2+

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The goal of this study was to investigate the isolated and combined effect of ebselen and Hg2+ on calcium influx and on glutamatergic system. We examined the in vitro effects of 2 phenyl-1,2-benzisoselenazol-3(2H)-ona), (Ebselen) on 45Ca2+ influx in synaptosomes of rat at rest and during depolarization and glutamate uptake into synaptosomes. Entry of 45Ca was measured during exposure to mercury in non-depolarizing and depolarizing solutions. Ebselen abolished the inhibition of 45Ca2+ influx on non-depolarizing conditions; however, ebselen did no modify inhibition uptake of 45Ca2+caused by Hg2+ in high K+ depolarizing medium. Ebselen did not modify glutamate uptake inhibition caused by Hg2+ in synaptosomes. These results indicate that ebselen has an in vitro protective effect against Hg2+ induced inhibition of Ca2+ influx into synaptosomes, depending on the depolarizing conditions of the assay. The effects of Hg2+ on glutamate uptake were not modified by ebselen, suggesting that its protection is dependent on the target protein considered.

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  1. Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, Brazil

    M. B. Moretto, J. Franco, T. Posser, C. W. Nogueira, G. Zeni & J. B. T. Rocha

  2. Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde; UFRGS Rua: Ramiro Barcelos, 2 600.anexo.CEP:90.035-003, Porto Alegre, RS, Brazil; E-mail:

    M. B. Moretto

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Moretto, M.B., Franco, J., Posser, T. et al. Ebselen Protects Ca2+ Influx Blockage But Does Not Protect Glutamate Uptake Inhibition Caused By Hg2+ . Neurochem Res 29, 1801–1806 (2004). https://doi.org/10.1023/B:NERE.0000042205.08917.f2

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