Abstract
Purpose : To study the correlation between the expression of integrin fractions and adhesion molecules on granulosa cells (GC) and follicular development.
Methods : GC and follicular fluid (FF) were obtained at oocyte retrieval for ICSI. Expression of adhesion molecules on GC was studied by flow cytometry. Statistics were evaluated using the Student t test and simple linear regression analysis.
Results : α5 integrin fraction was significantly (p<0.01) higher, while α6 fraction and CD9 were significantly (p<0.01 and p<0.001, respectively) lower in GC from FF with metaphase II oocytes. A direct significant correlation was observed between FF progesterone and the α5 expression on GC (r=0.54). In contrast, an inverse significant correlation was observed between FF progesterone level and the expression of α6 and CD9 (r= −0.40 and −0.41, respectively).
Conclusions : The expression pattern for integrin fractions and adhesion molecules may be of predictive value in assessing the state of differentiation of the human follicle.
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Clavero, A., Castilla, J.A., Martinez, L. et al. Expression of Integrin Fraction and Adhesion Molecules on Human Granulosa Cells and Its Relation with Oocyte Maturity and Follicular Steroidogenesis. J Assist Reprod Genet 21, 187–195 (2004). https://doi.org/10.1023/B:JARG.0000040233.32946.60
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DOI: https://doi.org/10.1023/B:JARG.0000040233.32946.60