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Plasma GLP-2 Levels and Intestinal Markers in the Juvenile Pig During Intestinal Adaptation: Effects of Different Diet Regimens

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Abstract

Adaptation of the residual small bowel following resection is dependent on luminal and humoral factors. We aimed to establish if circulating levels of glucagon-like peptide (GLP-2) change under different dietary regimens following resection and to determine if there is a relationship between plasma GLP-2 levels and markers of intestinal adaptation. Four-week-old piglets underwent a 75% proximal small bowel resection (n=31) or transection (n=14). Postoperatively they received either pig chow (n=14), nonpolymeric (elemental) infant formula (n=7), or polymeric infant formula alone (n=8) or supplemented either with fiber (n=6) or with bovine colostrum protein concentrate (CPC; n=10) for 8 weeks until sacrifice. Plasma GLP-2 levels were measured at weeks 0, 2, 4, and 8 postoperatively. In addition, end-stage parameters were studied at week 8 including weight gain, ileal villus height, crypt depth, and disaccharidase levels. Plasma GLP-2 levels were higher in resected animals compared to transected animals fed the same diet. Plasma GLP-2 levels were significantly increased in the colostrum protein isolate-supplemented animals following resection compared to all other diet groups. The increase in plasma GLP-2 (pM) was greatest in the first 2 weeks postresection (week 0, 15.5; week 2, 30.9), followed by a plateau at weeks 2 to 4 and a decrease in GLP-2 levels from week 4 to week 8. At week 8, no relationships were found between the plasma GLP-2 levels and the measurements of weight gain, villus height, lactase, sucrase, maltase, crypt depth, or villus/crypt ratio. Plasma GLP-2 levels increase in the first weeks following massive small intestinal resection. The increase in plasma GLP-2 levels was enhanced by supplementation of the diet with CPC. The changes in GLP-2 levels observed in this study may suggest that GLP-2 plays a role in the adaptive response in the intestine following resection in this preclinical model.

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Authors and Affiliations

  1. Department of Gastroenterology and Clinical Nutrition, Murdoch Childrens Research Institute, Royal Children's Hospital, Australia

    Monique C. Paris, Eva Nagy & Julie E. Binesm

  2. Prince Henry's Institute of Medical Research, Melbourne, Australia

    Peter J. Fuller

  3. Clinical Epidemiology & Biostatistics Unit, Royal Children's Hospital, Australia

    Bendix Carstensen

  4. Department of Surgery, Royal Children's Hospital, Australia

    Russell G. Taylor & Magdy Sourial

  5. Department of Medical Physiology, University of Copenhagen, Copenhagen, Denmark

    Jens J. Holst

  6. Department of Paediatrics, University of Melbourne, Australia

    Julie E. Binesm

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  1. Monique C. Paris
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  2. Peter J. Fuller
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  3. Bendix Carstensen
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  4. Eva Nagy
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Paris, M.C., Fuller, P.J., Carstensen, B. et al. Plasma GLP-2 Levels and Intestinal Markers in the Juvenile Pig During Intestinal Adaptation: Effects of Different Diet Regimens. Dig Dis Sci 49, 1688–1695 (2004). https://doi.org/10.1023/B:DDAS.0000043388.52260.2f

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  • DOI: https://doi.org/10.1023/B:DDAS.0000043388.52260.2f

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