Abstract
Recombinant endostatin was transiently expressed in Agrobacterium-inoculated leaf disks of Nicotiana tabacum var. Xanthi with a molecular size of 23 kDa. Expression of endostatin from a replicating vector based on tomato golden mosaic virus (TGMV) was 170% higher at the transcript level and double higher at the protein level than from a control vector of a non-replicating construct. Purified recombinant endostatin from tobacco leaf-disks has an anti-proliferative effect on bovine endothelial cells.
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Hong, S.H., Kim, K.I., Chung, H.Y. et al. Expression of recombinant endostatin in Agrobacterium-inoculated leaf disks of Nicotiana tabacum var. Xanthi. Biotechnology Letters 26, 1433–1439 (2004). https://doi.org/10.1023/B:BILE.0000045647.91071.40
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DOI: https://doi.org/10.1023/B:BILE.0000045647.91071.40