Abstract
Ageing is a complex phenomenon with possibly a multitude of contributions from many genes. In our study, we tested the degree of contribution of alcohol dehydrogenase (Adh) polymorphism (as specific enzyme activities) to ageing in Drosophila melanogaster. Our results indicate an overdominance of FS genotypes on ageing flies, with the two homozygotes, i.e., FF and SS, having significantly lower mean longevities. However, that kind of life-span–genotype relationship was not detected when the genotypes were observed for the immediate function of the Adhlocus, ADH enzyme activity in which mean ADH-FF activity was the highest as compared to those of ADH-FS and ADH-SS. This irrelevancy of the ADH enzyme function to ageing, with an obvious overdominance effect of Adh locus, is discussed considering Adh's known relation to other life history traits.
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References
Benyajati C, Wang N, Reddy A, Weinberg E and Sofer W (1980) Alcohol dehydrogenase in Drosophila: isolation and characterization of messenger RNA and cDNA clone. Nucleic Acids Res 8: 5649–5667
Bozcuk AN (1981) Genetics of longevity in DrosophilaV. The specific and hybridized effects of rolled, sepia, ebony and eyeless autosomal mutants. Exp Gerontol 16: 415–427
Chambers GK (1988) The Drosophila alcohol dehydrogenase gene-enzyme system. Advances in Genetics 25: 39–108
Durusoy M, Diril N and Bozcuk AN (1995a) Age-related activity of catalase in different genotypes of Drosophila melanogaster. Exp Gerontol 30: 77–86
Durusoy M, Diril N and Bozcuk AN (1995b) Age-related activity of glutathione S-transferase in three different genotypes of Drosophila melanogaster. Tr J Biology 19: 337–342
Gasperi G, Kafedzopoulos D, Christoulidou A, Bouriotis V and Savacis C (1994) Isolation and partial characterization of two alcohol dehydrogenase isozymes from the medfly Ceratitis capitata. Insect Biochem Molec Bio 24 (1): 87–94
Geer BW, Heinstra PWH and McKechnie SW (1993) The biological basis of ethanol tolerance in Drosophila. Comp Biochem Physiol 105B: 203–229
Kerver JWM and van Delden W (1985) Development of tolerance to ethanol in relation to the alcohol dehydrogenase locus in Drosophila melanogaster. Heredity 55: 355–368
Kreitman M (1983) Nucleotide polymorphism at the alcohol dehydrogenase locus of Drosophila melanogaster. Nature (London) 304: 412–417
Lowry OH, Rosenbrough NJ, Farr AL and Randall RJ (1951) Protein measurement with folin reagent. J Biol Chem 193: 265–275
Maroni G (1993) An Atlas of Drosophila Genes. Oxford University Press, New York, Oxford
Oudman L, van Delden W, Kamping A and Bijlsma R (1991) Polymorphism at Adh and aGpdh loci in Drosophila melanogaster: effects of rearing temperature on developmental rate, body weight, and some biochemical parameters. Heredity 67: 103–115
Oudman L, van Delden W, Kamping A and Bijlsma R (1992) Interaction between the Adh and aGpdh loci in Drosophila melanogaster: adult survival at high temperature. Heredity 68: 289–297
Pieragostini E, Sangiorgi S and Cavicchi S (1979) Adh, system and genetic background: interaction with wing length in Drosophila melanogaster. Genetica 50: 201–206
Sokal RR and Rohlf FJ (1995) Biometry. WH Freeman and Company, New York
Van Delden W (1982) The alcohol dehydrogenase polymorphism in Drosophila melanogaster: selection at an enzyme locus. Evol Biol 15: 187–222
Van Delden W and Kamping A (1980) The alcohol dehydrogenase polymorphism in populations of Drosophila melanogaster. IV. Survival at high temperature. Genetica 51: 179–185
Van Delden W and Kamping A (1997) Worldwide latitudinal clines for the alcohol dehydrogenase polymorphism in Drosophila melanogaster: what is the unit of selection? In: Bijlsma R and Loeschke V (eds) Environmental Stress, Adaptation and Evolution, pp. 97–115. Birkhauser Verlag, Basel
Winberg JO and McKinley-McKee JS (1994) Drosophila melanogaster alcohol dehydrogenase: product inhibition studies. Biochem J 301: 901–909
Zwaan BJ (1999) The evolutionary genetics of ageing and longevity. Heredity 82: 589–597
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Bozcuk, A., Sumer, S., Ozsoy, E. et al. Age-Related Enzyme Activity in Different Genotypes of Alcohol Dehydrogenase (ADH) in Drosophila Melanogaster . Biogerontology 5, 243–247 (2004). https://doi.org/10.1023/B:BGEN.0000038025.07744.75
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DOI: https://doi.org/10.1023/B:BGEN.0000038025.07744.75