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Differential Properties Between an Endogenous Brain Na+, K+-ATPase Inhibitor and Ouabain

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Abstract

By means of a Sephadex G-50 column and anionic exchange HPLC a cerebral cortex soluble fraction (II-E) which highly inhibits neuronal Na+-K+-ATPase activity has been previously obtained. Herein, II-E properties are compared with those of the cardenolide ouabain, the selective and specific Na+, K+-ATPase inhibitor. It was observed that alkali treatment destroyed II-E but not ouabain inhibitory activity. II-E presented a maximal absorbance at 265 nm both at pH 7 and pH 2 which diminished at pH 10. Ouabain showed a maximum at 220 nm which was not altered by alkalinization. II-E was not retained in a C-18 column, indicating its hydrophilic nature, whereas ouabain presented a 26-min retention time in reverse phase HPLC. Therefore, it is concluded that the inhibitory factor present in II-E is structurally different to ouabain.

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REFERENCES

  1. Stahl, W. L. 1986. The Na+, K+-ATPase of nervous tissue. Neurochem. Int. 4:449–476.

    Google Scholar 

  2. Wu, P. H. 1986. Na+, K+-ATPase in nervous tissue. Pages 451–502, in Boulton, A. A., Baker, G. B., and Wu, P. H. (eds.), Neuromethods, Enzymes, Humana, Clifton, NJ.

    Google Scholar 

  3. Rodriguez de Lores Arnaiz, G., and Mistrorigo de Pacheco, M. 1978. Regulation of (Na+, K+) adenosine triphosphatase of nerve ending membranes: action of norepinephrine and a soluble factor. Neurochem. Res. 3:733–744.

    Google Scholar 

  4. Rodriguez de Lores Arnaiz, G., and Antonelli de Gómez de Lima, M. 1988. The aging of a brain soluble fraction modifies its effect on the activity of neuronal Na+, K+-ATPase. Life Sci. 42:2683–2689.

    Google Scholar 

  5. Rodríguez de Lores Arnaiz, G., and Antonelli de Gómez de Lima, M. 1986. Partial characterization of an endogenous factor which modulates the effect of catecholamines on synaptosomal Na+, K+-ATPase. Neurochem. Res. 11:933–947.

    Google Scholar 

  6. Rodríguez de Lores Arnaiz, G., Antonelli de Gómez de Lima, M., and Girardi, E. 1988. Different properties of two brain fractions separated in Sephadex G-50 that modify synaptosomal ATPase activities. Neurochem. Res. 3:229–235.

    Google Scholar 

  7. Nowicki, S., Enero, M. A., and Rodríguez de Lores Arnaiz, G. 1990. Diuretic and natriuretic effect of a brain soluble fraction that inhibits neuronal Na+, K+-ATPase. Life Sci. 47:1091–1098.

    Google Scholar 

  8. Rodríguez de Lores Arnaiz, G., and Pellegrino de Iraldi, A. 1991. The release of catecholamines by an endogenous factor that inhibits neuronal Na+, K+-ATPase. Micr. Electr. Biol. Cell. 15:93–106.

    Google Scholar 

  9. Antonelli, M., Casillas, T., and Rodríguez de Lores Arnaiz, G. 1991. Effect of Na+, K+ ATPase modifiers on high-affinity ouabain binding determined by quantitative autoradiography. J. Neurosci. Res 28:342–331.

    Google Scholar 

  10. Rodríguez de Lores Arnaiz, G., and Peña, C. 1995. Characterization of synaptosomal membrane Na+, K+-ATPase inhibitors. Neurochem. Int. 27:319–327.

    Google Scholar 

  11. Rodríguez de Lores Arnaiz, G., Alberici, M., and De Robertis, E. 1967. Ultrastructural and enzymic studies of cholinergic and non-cholinergic synpatic membranes isolated from brain cortex. J. Neurochem. 14:215–225.

    Google Scholar 

  12. Albers, R. W., Rodríguez de Lores Arnaiz, G., and De Robertis, E. 1965. Sodium-potassium-activated ATPase and potassium-activated p-nitrophenylphosphatase: a comparison of their subcellular localizations in rat brain. Proc. Natl. Acad. Sci. USA 53:557–564.

    Google Scholar 

  13. Lowry, O. H., Rosebrough., N. J., Farr, A. L., and Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.

    Google Scholar 

  14. Schwartz, A., Lindenmayer, G. E., and Allen, J. C. 1975. The sodium potassium adenosine triphosphatase: pharmacological, physiological and biochemical aspects. Pharmacol. Rev. 27:3–134.

    Google Scholar 

  15. Goto, A., Yamada, K., Yagi, N., Yoshioka, M., and Sugimoto, T.: Physiology and pharmacology of endogenous digitalis-like factors. Pharmacol. Rev. 44:377–399.

  16. Rodríguez de Lores Arnaiz, G. 1992. In search of synaptosomal Na+, K+-ATPase regulators. Mol. Neurobiol. 6:359–375.

    Google Scholar 

  17. Hamlyn, J. M., Blaustein, M. P., Bova, S. DuCharme, D. W., Harris, D. W., Mandel F., Mathews, W. R., and Ludens, J. H. 1991. Identification and characterization of a ouabain-like compound from human plasma. Proc. Natl. Acad. Sci. USA 88:6259–6263.

    Google Scholar 

  18. Mathews, W. R., DuCharme, D. W., Hamlyn, J. M., Harris, D. W., Mandel, F., Clark, M. A., and Ludens, J. H. 1991. Mass spectral characterization of an endogenous digitalis-like factor from human plasma. Hypertension 17:930–935.

    Google Scholar 

  19. Balzan, S., Ghione, S., Pieraccini, L., Biver, P., Di Bartolo, V., and Montali, U. 1994. Endogenous digitalis-like factor from umbilical cord and ouabain: comparison of biochemical properties. Pages 755–758, in Bamberg, E., and Schoner, W., (eds.), The Sodium Pump, Steinkopff, Darmstadt.

    Google Scholar 

  20. Di Bartolo, V., Balzan, S., Pieraccini, L., Ghione, S., Pegoraro, S., Biver, P., Revoltella, R., and Montali, U. 1995. Evidence for an endogenous ouabain-like immunoreactive factor in human newborn plasma coeluted with ouabain on HPLC. Life Sci. 57:1417–1425.

    Google Scholar 

  21. Tamura, M., Lam, T-T., and Inagami, T. 1988. Isolation and characterization of a specific endogenous Na+, K+-ATPase inhibitor from bovine adrenal. Biochemistry 27:4244–4253.

    Google Scholar 

  22. Goto, A., Ishiguro, T., Yamada, K., Ishii, M., Yoshioka, M., Eguchi, C., Shimora, M., and Sugimoto, T. 1990. Isolation of a urinary digitalis-like factor indistinguishable from digoxin. Biochem. Biophys. Res. Commun. 173:1093–1101.

    Google Scholar 

  23. Tymiak, A. A., Norman, J. A., Bolgar, M., DiDonato, G. C., Lee, H., Parker, W. L., Lo, L-C., Berova, N., Nakanishi, K., Haber, E., and Haupert, G. T., Jr. 1993. Physicochemical characterization of a ouabain isomer isolated from bovine hypothalamus. Proc. Natl. Acad. Sci. USA 90:8189–8193.

    Google Scholar 

  24. Tamura, M., Harris, T. M., Konishi, F., and Inagami, T. 1993. Isolation and characterization of an endogenous Na+, K+-ATPase-specific inhibitor from pig urine. Eur. J. Biochem. 211:317–327.

    Google Scholar 

  25. Longerich, L., Johnson, E., and Gault, M. H. 1993. Digoxin-like factors in herbal teas. Clin. Invest. Med. 16:210–218.

    Google Scholar 

  26. Rodríguez de Lores Arnaiz, G. 1993. An endogenous factor which interacts with synaptosomal membrane Na+, K+-ATPase activation by K+. Neurochem. Res. 18:655–661.

    Google Scholar 

  27. Hamlyn, J. M., Ringel, R., Schaeffer, J., Levinson, P. D., Hamilton, B. P., Kowarski, A. A., and Blaustein, M. P. 1982. A circulating inhibitor of (Na+, K+) ATPase associated with essential hypertension. Nature 300:650–652.

    Google Scholar 

  28. Kunes, J., Stolba, P., Pohlova, I., Jelinek, J., and Zicha, J. 1985. The importance of endogenous digoxin-like factors in rats with various forms of experimental hypertension. Clin. Exp. Hypert. A7:707.

    Google Scholar 

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Authors and Affiliations

  1. Instituto de Química y Fisicoquímica Biológicas, Facultad de Farmacia y Bioquímica, Junín 956, 1113-, Buenos Aires, Argentina

    Clara Peña

  2. Instituto de Biología Celular y Neurociencias “Prof. Eduardo De Robertis”, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, 1121-, Buenos Aires, Argentina

    Georgina Rodríguez de Lores Arnaiz

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  1. Clara Peña
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  2. Georgina Rodríguez de Lores Arnaiz
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Peña, C., de Lores Arnaiz, G.R. Differential Properties Between an Endogenous Brain Na+, K+-ATPase Inhibitor and Ouabain. Neurochem Res 22, 379–383 (1997). https://doi.org/10.1023/A:1027343323629

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  • DOI: https://doi.org/10.1023/A:1027343323629

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