Skip to main content
SpringerLink
Log in

Use of Green Fluorescent Protein (GFP) and Its Homologs for in vivo Protein Motility Studies

  • Published:
Biochemistry (Moscow) Aims and scope Submit manuscript

Abstract

Green fluorescent protein (GFP) and its homologs are widely used as fluorescent markers of gene expression and for determination of protein localization and motility in living cells. In particular, based on GFP and GFP-like proteins a number of techniques have been developed that can be used either to estimate protein mobility in living cells, or to introduce a distinctive fluorescent signal in order to track the movement of labeled molecules directly. Considerable progress in the development of such technologies in the last two or three years motivates us to reevaluate the present scope of biotechnological instruments in studies of protein movement in cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Prasher, D. C., Eckenrode, V. K., Ward, W. W., Prendergast, F. G., and Cormier, M. J. (1992) Gene, 111, 229-233.

    Google Scholar 

  2. Yang, F., Moss, L. G., and Phillips, G. N. (1996) Nat. Biotechnol., 14, 1246-1251.

    Google Scholar 

  3. Ormo, M., Cubitt, A. B., Kallio, K., Gross, L. A., Tsien, R. Y., and Remington, S. J. (1996) Science, 273, 1392-1395.

    Google Scholar 

  4. Patterson, G., Day, R. N., and Piston, D. (2001) J. Cell Sci., 114, 837-838.

    Google Scholar 

  5. Matz, M. V., Fradkov, A. F., Labas, Y. A., Savitsky, A. P., Zaraisky, A. G., Markelov, M. L., and Lukyanov, S. A. (1999) Nat. Biotechnol., 17, 969-973.

    Google Scholar 

  6. Fradkov, A. F., Chen, Y., Ding, L., Barsova, E. V., Matz, M. V., and Lukyanov, S. A. (2000) FEBS Lett., 479, 127-130.

    Google Scholar 

  7. Wiedenmann, J., Schenk, A., Rocker, C., Girod, A., Spindler, K. D., and Nienhaus, G. U. (2002) Proc. Natl. Acad. Sci. USA, 99, 11646-11651.

    Google Scholar 

  8. Gurskaya, N. G., Fradkov, A. F., Terskikh, A., Matz, M. V., Labas, Y. A., Martynov, V. I., Yanushevich, Y. G., Lukyanov, K. A., and Lukyanov, S. A. (2001) FEBS Lett., 507, 16-20.

    Google Scholar 

  9. Baird, G. S., Zacharias, D. A., and Tsien, R. Y. (1999) Proc. Natl. Acad. Sci. USA, 96, 11241-11246.

    Google Scholar 

  10. Nagai, T., Sawano, A., Park, E. S., and Miyawaki, A. (2001) Proc. Natl. Acad. Sci. USA, 13, 3197-3202.

    Google Scholar 

  11. Miyawaki, A., Llopis, J., Heim, R., McCaffery, J. M., Adams, J. A., Ikura, M., and Tsien, R. Y. (1997) Nature, 28, 882-887.

    Google Scholar 

  12. Miesenbock, G., de Angelis, D. A., and Rothman, J. E. (1998) Nature, 9, 192-195.

    Google Scholar 

  13. Llopis, J., McCaffery, J. M., Miyawaki, A., Farquhar, M. G., and Tsien, R. Y. (1998) Proc. Natl. Acad. Sci. USA, 95, 6803-6808.

    Google Scholar 

  14. Elsliger, M. A., Wachter, R. M., Hanson, G. T., Kallio, K., and Remington, S. J. (1999) Biochemistry, 38, 5296-5301.

    Google Scholar 

  15. Siegel, M. S., and Isacoff, E. Y. (1997) Neuron, 19, 735-741.

    Google Scholar 

  16. White, J., and Stelzer, E. (1999) Trends Cell Biol., 9, 61-65.

    Google Scholar 

  17. Lippincott-Schwartz, J., Snapp, E., and Kenworthy, A. (2001) Nat. Rev. Mol. Cell. Biol., 2, 444-456.

    Google Scholar 

  18. Patterson, G. H., Knobel, S. M., Sharif, W. D., Kain, S. R., and Piston, D. W. (1997) Biophys. J., 73, 2782-2790.

    Google Scholar 

  19. Gaietta, G., Deerinck, T. J., Adams, S. R., Bouwer, J., Tour, O., Laird, D. W., Sosinsky, G. E., Tsien, R. Y., and Ellisman, M. H. (2002) Science, 296, 503-507.

    Google Scholar 

  20. Dunn, G. A., Dobbie, I. M., Monypenny, J., Holt, M. R., and Zicha, D. (2002) J. Microsc., 205, 109-112.

    Google Scholar 

  21. Garcia-Parajo, M. F., Koopman, M., van Dijk, E. M., Subramaniam, V., and van Hulst, N. F. (2001) Proc. Natl. Acad. Sci. USA, 98, 14392-14397.

    Google Scholar 

  22. Cotlet, M., Hofkens, J., Habuchi, S., Dirix, G., van Guyse, M., Michiels, J., Vanderleyden, J., and de Schryver, F. C. (2001) Proc. Natl. Acad. Sci. USA, 98, 14398-14403.

    Google Scholar 

  23. Marchant, J. S., Stutzmann, G. E., Leissring, M. A., LaFerla, F. M., and Parker, I. (2001) Nat. Biotechnol., 19, 645-649.

    Google Scholar 

  24. Ando, R., Hama, H., Yamamoto-Hino, M., Mizuno, H., and Miyawaki, A. (2002) Proc. Natl. Acad. Sci. USA, 99, 12651-12656.

    Google Scholar 

  25. Chudakov, D. M., Belousov, V. V., Zaraisky, A. G., Novoselov, V. V., Staroverov, D. B., Zorov, D. B., Lukyanov, S. A., and Lukyanov, K. A. (2003) Nat. Biotechnol., 21, 191-194.

    Google Scholar 

  26. Chudakov, D. M., Feofanov, A. V., Mudrik, N. N., Lukyanov, S. A., and Lukyanov, K. A. (2002) J. Biol. Chem., 278, 7215-7219.

    Google Scholar 

  27. Campbell, R. E., Tour, O., Palmer, A. E., Steinbach, P. A., Baird, G. S., Zacharias, D. A., and Tsien, R. Y. (2002) Proc. Natl. Acad. Sci. USA, 99, 7877-7882.

    Google Scholar 

  28. Fradkov, A. F., Verkhusha, V. V., Staroverov, D. B., Bulina, M. E., Yanushevich, Y. G., Martynov, V. I., Lukyanov, S. A., and Lukyanov, K. A. (2002) Biochem. J., 368, 17-21.

    Google Scholar 

  29. Bulina, M. E., Verkhusha, V. V., Staroverov, D. B., Chudakov, D. M., and Lukyanov, K. A. (2002) Biochem. J., 371, 109-114.

    Google Scholar 

  30. Gavin, P., Devenish, R. J., and Prescott, M. (2002) Biochem. Biophys. Res. Commun., 298, 707-713.

    Google Scholar 

  31. Elowitz, M. B., Surette, M. G., Wolf, P. E., Stock, J., and Leibler, S. (1997) Curr. Biol., 7, 809-812.

    Google Scholar 

  32. Sawin, K. E., and Nurse, P. (1997) Curr. Biol., 7, 606-607.

    Google Scholar 

  33. Elowitz, M. B., Surette, M. G., Wolf, P. E., Stock, J. B., and Leibler, S. (1999) J. Bacteriol., 181, 197-203.

    Google Scholar 

  34. Chattoraj, M., King, B. A., Bublitz, G. U., and Boxer, S. G. (1996) Proc. Natl. Acad. Sci. USA, 93, 8362-8367.

    Google Scholar 

  35. Yokoe, H., and Meyer, T. (1996) Nat. Biotechnol., 14, 1252-1256.

    Google Scholar 

  36. Patterson, G. H., and Lippincott-Schwartz, J. (2002) Science, 297, 1873-1877.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, 117997, Russia

    D. M. Chudakov & K. A. Lukyanov

Authors
  1. D. M. Chudakov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. A. Lukyanov
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chudakov, D.M., Lukyanov, K.A. Use of Green Fluorescent Protein (GFP) and Its Homologs for in vivo Protein Motility Studies. Biochemistry (Moscow) 68, 952–957 (2003). https://doi.org/10.1023/A:1026048109654

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1026048109654

Search

Navigation