Abstract
cDNA encoding betaine aldehyde dehydrogenase (BADH) from the halophyte Suaeda liaotungensis has been cloned, sequenced and expressed in tobacco (Nictiana tabacum 89). The full-length cDNA is 1506 base pairs (bp) long and encodes a 502 amino-acid polypeptide. The cDNA fragment coding for the mature enzyme was cloned into vector pCAMBIA-1301 for expression in tobacco. Southern blotting analysis showed that that the Badh gene was integrated into the genome of tobacco. Tobacco expressing BADH survived on MS medium containing 200 mM NaCl, whereas the untransformed plants turned yellow after about 20 d and died.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Guo BH, Zhang YM, Li HJ, Du LQ, Li YX, Zhang JS, Chen SY, Zhu ZQ (2000) Transformation of wheat with a gene encoding for the betaine aldehyde dehydrogenase. Acta Bot. Sin. 42: 279–283.
Hibino T, Meng YL, Kawamitsu Y, Uehara N, Matsuda N, Tanaka Y, Ishikawa H, Bana S, Takabe T, Wafda K, Ishii T, Takabe T (2001) Molecular cloning and functional characterization of two kinds of betaine-aldehyde dehydrogenase in betaineaccumulating mangrove Avicennia marina (Forsk.) Vierh. Plant Mol. Biol. 45: 353–363.
Huang J, Hirji R, Adam L, Rozwadowski KL, Hammerlindl JK, Keller WA, Selvaraj G (2000) Genetic engineering of glycinebetaine production toward enhancing stress tolerance in plants: metabolic limitations. Plant Physiol. 122: 747–756.
Legaria J, Rajsbaum R, Munoz-Clares RA, Villegas-Sepulveda N, Simpson J, Iturriaga G (1998) Molecular characterization of two genes encoding betaine aldehyde dehydrogenase from amaranth. Expression in leaves under short-term exposure to osmotic stress or abscisic acid. Gene. 218: 69–76.
Li YX, Chang FQ, Du LQ, Guo BH, Li HJ, Zhang JS, Chen SY, Zhu ZQ (2000) Genetic Transformation of watercress with a gene encoding for betaine-aldehyde Dehydrogenase (BADH). Acta Bot. Sin. 42: 480–484.
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Plant Physiol. 15: 473–497.
Rathinasabapathi B, McCue KF, Gage DA, Handson AD (1994) Metabolic engineering of glycine betaine synthesis: plant betaine aldehyyde dehydrogenases lacking typical transit peptides are targeted to tobacco chloroplasts where they confer betaine aldehyde resistance. Planta 193: 155–162.
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. New York: Cold Spring Harbor Laboratory Press, pp. 456–495.
Tang ZC (1999) Modern Experiment Procotols in Plant Physiology. Beijing: Science Press, pp. 302–308.
Wang GL, Fang HJ (1998) Principles and Technology of Plant Gene Engineering. Beijing: Science Press, pp. 552–553.
Weretilnyk EA, Hanson AD (1990) Molecular cloning of a plant betaine-aldehyde dehydrogenase, an enzyme implicated in adaptation to salinity and drought. Proc. Natl. Acad. Sci. USA 87: 2745–2749.
Weretilnyk EA, Hanson AD (1992) Salt-inducible betaine aldehyde dehydrogenase from sugar beet: cDNA cloning and expression. Plant Mol. Biol. 18: 1–11.
Xiao G, Zhang GY, Liu FH, Wang J, Chen SY, Li C, Geng HZ (1995) Study on betaine aldehyde dehydrogenase of Atriplex hortensis L. Chin. Sci. Bull. 40: 741–745.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Li, QL., Gao, XR., Yu, XH. et al. Molecular cloning and characterization of betaine aldehyde dehydrogenase gene from Suaeda liaotungensis and its use in improved tolerance to salinity in transgenic tobacco. Biotechnology Letters 25, 1431–1436 (2003). https://doi.org/10.1023/A:1025003628446
Issue Date:
DOI: https://doi.org/10.1023/A:1025003628446