Manipulating anthocyanin composition in Vitis vinifera suspension cultures by elicitation with jasmonic acid and light irradiation

Abstract

Jasmonic acid altered the accumulation of major anthocyanins in Vitis vinifera cell culture. Peonidin 3-glucoside content at day three was increased from 0.3 to 1.7 mg g⁻¹ dry cell wt while other major anthocyanins were increased by smaller increments. By day 14, the content of methylated and acylated anthocyanins (peonidin 3-p-coumaroylglucoside and malvidin 3-p-coumaroylglucoside) was 6.3 mg g⁻¹ DCW, in response to treatment with jasmonic acid, and comprising ∼45% (w/w) of total anthocyanins. In comparison, the untreated control culture contained 1.2 mg g⁻¹ DCW which made up ∼32% (w/w) of total anthocyanins. Light further enhanced anthocyanin accumulation induced by jasmonic acid elicitation. The content of peonidin 3-glucoside at day 3 was 6.6 mg g⁻¹ DCW, 22-fold higher than control cultures while the content in response to light irradiation alone was 0.6 mg g⁻¹ DCW. When a highly pigmented cell line was elicited with jasmonic acid total anthocyanins increased from 9.2 to 20.7 mg g⁻¹ DCW, but there was no change in the anthocyanin composition.