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Dictyostelium discoideum adhesion and motility under shear flow: experimental and theoretical approaches

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Abstract

Among the different assays to measure cell adhesion, shear-flow detachment chambers offer the advantage to study both passive and active aspects of the phenomena on large cell numbers. Mathematical modeling allows full exploitation of the data by relating molecular parameters to cell mechanics. Using D. discoideum as a model system, we explain how cell detachment kinetics gives access to the rate constants describing the passive association or dissociation of the cell membrane to a given substrate.

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References

  • Bell GI (1978) Models for the specific adhesion of cells to cells. Science 200: 618–627.

    Google Scholar 

  • Bell GI, Dembo M and Bongrand P (1984) Cell adhesion: competition between nonspecific repulsion and specific bonding. Biophys J 45: 1051–1064.

    Google Scholar 

  • Benoit M, Gabriel D, Gerisch G and Gaub HE (2000) Discrete interactions in cell adhesion measured by single-molecule force spectroscopy. Nat Cell Biol 2: 313–317.

    Google Scholar 

  • Bozzaro S and Roseman S (1983a) Adhesion of Dictyostelium discoideum cells to carbohydrates immobilized in polyacrylamide gels: I. Evidence for three sugar-specific cell surface receptors. J Biol Chem 258: 13,882–13,889.

    Google Scholar 

  • Bozzaro S and Roseman S (1983b) Adhesion of Dictyostelium discoideum cells to carbohydrates immobilized in polyacrylamide gels: II. Effect of D-glucoside derivatives on development. J Biol Chem 258: 13,890–13,897.

    Google Scholar 

  • Chadwick CM, Ellison JE and Garrod DR (1984) Dual role for Dictyostelium contact site B in phagocytosis and developmental size regulation. Nature 307: 646–647.

    Google Scholar 

  • Chia CP (1996) A 130-kDa plasma membrane glycoprotein involved in Dictyostelium phagocytosis. Exp Cell Res 227: 182–189.

    Google Scholar 

  • Coates JC, Grimson MJ, Williams RS, Bergman W, Blanton RL and Harwood AJ (2002) Loss of the beta-catenin homologue aardvark causes ectopic stalk formation in Dictyostelium. Mech Dev 116: 117–127.

    Google Scholar 

  • Coates JC and Harwood AJ (2001) Cell-cell adhesion and signal transduction during Dictyostelium development. J Cell Sci 114: 4349–4358.

    Google Scholar 

  • Cornillon S, Pech E, Benghezal M, Ravanel K, Gaynor E, Letourneur F, Bruckert F and Cosson P (2000) Phg1p is a nine-transmembrane protein superfamily member involved in Dictyostelium adhesion and phagocytosis. J Biol Chem 275: 34,287–34,292.

    Google Scholar 

  • Cozens-Roberts C, Quinn JA and Lauffenberger DA (1990) Receptormediated adhesion phenomena. Model studies with the radial-flow detachment assay. Biophys J 58: 107–125.

    Google Scholar 

  • Decave E, Garrivier D, Brechet Y, Bruckert F and Fourcade B (2002a) Peeling process in living cell movement under shear flow. Phys Rev Lett 89: 108,101.

    Google Scholar 

  • Decave E, Garrivier D, Brechet Y, Fourcade B and Bruckert F (2002b) Shear flow-induced detachment kinetics of Dictyostelium discoideum cells from solid substrate. Biophys J 82: 2383–2395.

    Google Scholar 

  • Dembo M, Torney DC, Saxman K and Hammer D (1988) The reaction-limited kinetics of membrane-to-surface adhesion and detachment. Proc R Soc Lond B Biol Sci 234: 55–83.

    Google Scholar 

  • Desbarats L, Brar SK and Siu CH (1994) Involvement of cell-cell adhesion in the expression of the cell cohesion molecule gp80 in Dictyostelium discoideum. J Cell Sci 107: 1705–1712.

    Google Scholar 

  • Elie R and de Gennes PG (1994) The adhesion between elastomers. In: Rabin Y and Bruisnma R (ed.) Soft Order in Physical Systems. (pp. 57–71) Plenum Press, New York.

    Google Scholar 

  • Evans E (1998) Introductory lecture: energy landscapes of biomolecular adhesion and receptor anchoring at interfaces explored with dynamic force spectroscopy. Faraday Discussions 111: 1–16.

    Google Scholar 

  • Evans E, Ritchie K and Merkel R (1995) Sensitive force technique to probe molecular adhesion and structural linkages at biological interfaces. Biophys J 68: 2580–2587.

    Google Scholar 

  • Faix J, Gerisch G and Noegel AA (1990) Constitutive overexpression of the contact site A glycoprotein enables growth-phase cells of Dictyostelium discoideum to aggregate. Embo J 9: 2709–2716.

    Google Scholar 

  • Francis GW, Fisher LR, Gamble RA and Gingell D (1987) Direct measurement of cell detachment force on single cells using a new electromechanical method. J Cell Sci 87: 519–523.

    Google Scholar 

  • Fukui Y, Uyeda TQ, Kitayama C and Inoue S (2000) How well can an amoeba climb? Proc Natl Acad Sci USA 97: 10,020–10,025.

    Google Scholar 

  • Garrivier D, Decave E, Brechet Y, Bruckert F and Fourcade B (2002) Peeling model for cell detachement. Eur Phys J part E 8: 79–97.

    Google Scholar 

  • Gingell D (1981) The interpretation of interference-re.ection images of spread cells: signi.cant contributions from thin peripheral cytoplasm. J Cell Sci 49: 237–247.

    Google Scholar 

  • Grimson MJ, Coates JC, Reynolds JP, Shipman M, Blanton RL and Harwood AJ (2000) Adherens junctions and beta-catenin-mediated cell signalling in a non-metazoan organism. Nature 408: 727–731.

    Google Scholar 

  • Harloff C, Gerisch G and Noegel AA (1989) Selective elimination of the contact site A protein of Dictyostelium discoideum by gene disruption. Genes Dev 3: 2011–2019.

    Google Scholar 

  • Knecht DA, Fuller DL and Loomis WF (1987) Surface glycoprotein, gp24, involved in early adhesion of Dictyostelium discoideum. Dev Biol 121: 277–283.

    Google Scholar 

  • Leckband DE, Schmitt FJ, Israelachvili JN and Knoll W (1994) Direct force measurements of speci.c and nonspecific protein interactions. Biochemistry 33: 4611–4624.

    Google Scholar 

  • Maniak M, Rauchenberger R, Albrecht R, Murphy J and Gerisch G (1995) Coronin involved in phagocytosis: dynamics of particleinduced relocalization visualized by a green fluorescent protein Tag. Cell 83: 915–924.

    Google Scholar 

  • Muller K and Gerisch G (1978) A specific glycoprotein as the target site of adhesion blocking Fab in aggregating Dictyostelium cells. Nature 274: 445–449.

    Google Scholar 

  • Niewohner J, Weber I, Maniak M, Muller-Taubenberger A and Gerisch G (1997) Talin-null cells of Dictyostelium are strongly defective in adhesion to particle and substrate surfaces and slightly impaired in cytokinesis. J Cell Biol 138: 349–361.

    Google Scholar 

  • Pierres A, Benoliel AM, Bongrand P and van der Merwe PA (1996) Determination of the lifetime and force dependence of interactions of single bonds between surface-attached CD2 and CD48 adhesion molecules. Proc Natl Acad Sci USA 93: 15,114–15,118.

    Google Scholar 

  • Pierres A, Benoliel AM, Bongrand P and van der Merwe PA (1997) The dependence of the association rate of surface-attached adhesion molecules CD2 and CD48 on separation distance. FEBS Lett 403: 239–244.

    Google Scholar 

  • Pierres A, Feracci H, Delmas V, Benoliel AM, Thiery JP and Bongrand P (1998) Experimental study of the interaction range and association rate of surface-attached cadherin 11. Proc Natl Acad Sci USA 95: 9256–9261.

    Google Scholar 

  • Ponte E, Bracco E, Faix J and Bozzaro S (1998) Detection of subtle phenotypes: the case of the cell adhesion molecule csA in Dictyostelium. Proc Natl Acad Sci USA 95: 9360–9365.

    Google Scholar 

  • Riveline D, Zamir E, Balaban NQ, Schwarz US, Ishizaki T, Narumiya S, Kam Z, Geiger B and Bershadsky AD (2001) Focal contacts as mechanosensors: externally applied local mechanical force induces growth of focal contacts by an mDia1-dependent and ROCKindependent mechanism. J Cell Biol 153: 1175–1186.

    Google Scholar 

  • Simson R, Wallraff E, Faix J, Niewohner J, Gerisch G and Sackmann E (1998) Membrane bending modulus and adhesion energy of wild-type and mutant cells of Dictyostelium lacking talin or cortexillins. Biophys J 74: 514–522.

    Google Scholar 

  • Smith MJ, Berg EL and Lawrence MB (1999) A direct comparison of selectin-mediated transient, adhesive events using high temporal resolution. Biophys J 77: 3371–3383.

    Google Scholar 

  • Thoumine O, Ott A and Louvard D (1996) Critical centrifugal forces induce adhesion rupture or structural reorganization in cultured cells. Cell Motil Cytoskeleton 33: 276–287.

    Google Scholar 

  • Tuxworth RI, Weber I, Wessels D, Addicks GC, Soll DR, Gerisch G and Titus MA (2001) A role for myosin VII in dynamic cell adhesion. Curr Biol 11: 318–329.

    Google Scholar 

  • Vogel G, Thilo L, Schwarz H and Steinhart R (1980) Mechanism of phagocytosis in Dictyostelium discoideum: phagocytosis is mediated by different recognition sites as disclosed by mutants with altered phagocytotic properties. J Cell Biol 86: 456–465.

    Google Scholar 

  • Weber I, Wallra. E, Albrecht R and Gerisch G (1995) Motility and substratum adhesion of Dictyostelium wild-type and cytoskeletal mutant cells: a study by RICM/bright-field double-view image analysis. J Cell Sci 108: 1519–1530.

    Google Scholar 

  • Wong EF, Brar SK, Sesaki H, Yang C and Siu CH (1996) Molecular cloning and characterization of DdCAD-1, a Ca2+-dependent cell-cell adhesion molecule, in Dictyostelium discoideum. J Biol Chem 271: 16,399–16,3408.

    Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Biochimie et Biophysique des Systèmes Intégrés (UMR 5092), Département Réponse et Dynamique Cellulaires, CEA-Grenoble, 17 rue des Martyrs, 38054, Grenoble Cedex 09, France

    Franz Bruckert, Emmanuel Décavé & Michel Satre

  2. Structures et Propriétés des Architectures Moléculaires (UMR 5819), Département de Recherche Fondamentale sur la Matière Condensée, CEA-Grenoble, 38054, Grenoble Cedex 09, France

    Emmanuel Décavé, Daniel Garrivier & Bertrand Fourcade

  3. Département de Morphologie, Université de Genève, Centre Médical Universitaire, 1211 Geneva 4, Switzerland

    Pierre Cosson

  4. Laboratoire de Thermodynamique et de Physico-Chimie du Métal (UMR 5614), ENS d'Electrochimie Electrométallurgie, Domaine Universitaire, 38402, Saint-Martin d'Hères, France

    Yves Bréchet

Authors
  1. Franz Bruckert
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  2. Emmanuel Décavé
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  3. Daniel Garrivier
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  4. Pierre Cosson
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  5. Yves Bréchet
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  6. Bertrand Fourcade
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  7. Michel Satre
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Bruckert, F., Décavé, E., Garrivier, D. et al. Dictyostelium discoideum adhesion and motility under shear flow: experimental and theoretical approaches. J Muscle Res Cell Motil 23, 651–658 (2002). https://doi.org/10.1023/A:1024407107588

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  • DOI: https://doi.org/10.1023/A:1024407107588

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