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Synchrotron-based Biological Microspectroscopy: From the Mid-Infrared through the Far-Infrared Regimes

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Abstract

Infrared radiation from synchrotron storagerings serves as a high-brightness source fordiffraction-limited microspectroscopy inboth the mid- and far-infrared spectralranges. Mid-infrared absorption, due to localvibrational modes within complex molecules,is shown to be sensitive to small chemicalchanges associated with certain diseases.Farinfrared modes are believed to result from thefolding or twisting of larger, morecomplex molecules. The ability for thesynchrotron source to perform microscopy ata frequency of 1 THz is demonstrated.

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References

  1. Jackson, M., Mantsch, H.H.:Encyclopedia of Analytical Chemistry, Wiley & Sons, Chichester, Sussex, UK, 2000.

    Google Scholar 

  2. Choo, L.P., Mansfield, J.R., Pizzi, M., Somorjai, R.L., Jackson, M., Halliday, W.C. and Mantsch, H.H.: Infrared Spectra of Human Central Nervous System Tissue: Diagnosis of Alzheimer's Disease by Multivariate Analyses,Biospectroscopy 1 (1995), 141-148.

  3. Choo, L.P., Wetzel, D.L., Halliday, W.C., Jackson, M., LeVine, S.M. and Mantsch, H.H.:In situ Characterization of Beta-Amyloid in Alzheimer's Diseased Tissue by Synchrotron Fourier Transform Infrared Microspectroscopy,Biophys. J. 71 (1996), 1672-1679.

  4. Chiriboga, L., Xie, P., Yee, H., Zarou, D., Zakim, D. and Diem, M.: Infrared Spectroscopy of Human Tissue. IV. Detection of Dysplastic and Neoplastic Changes of Human Cervical Tissue via Infrared Microscopy,Cell Mol. Biol. (Noisy-le-grand) 44 (1998), 219-229.

  5. Lowry, S.R.: The Analysis of Exfoliated Cervical Cells by Infrared Microscopy,Cell Mol. Biol. (Noisy-le-grand) 44 (1998), 169-177.

  6. Liu, K.Z., Dixon, I.M. and Mantsch, H.H.: Distribution of Collagen Deposition in Cardiomyopathic Hamster Hearts Determined by Infrared Microscopy [In Process Citation],Cardiovasc. Pathol. 8 (1999), 41-47.

  7. Paschalis, E.P., Jacenko, O., Olsen, B., deCrombrugghe, B. and Boskey, A.L.: The Role of Type × Collagen in Endochondral Ossification as deduced by Fourier Transform Infrared Microscopy Analysis,Connect. Tissue Res. 35 (1996), 371-377.

  8. Camacho, N.P., Landis, W.J. and Boskey, A.L.: Mineral Changes in a Mouse Model of Osteogenesis Imperfecta detected by Fourier Transform Infrared Microscopy,Connect. Tissue Res. 35 (1996), 259-265.

  9. Miller, L.M., Hamerman, D., Chance, M.R. and Carlson, C.S.: Chemical Differences in Subchondral Osteoarthritic Bone Observed with Synchrotron Infrared Microspectroscopy,SPIE 3775 (1999), 104-112.

  10. Miller, L.M., Tibrewala, J. and Carlson, C.S.: Examination of Bone Chemical Composition in Osteoporosis Using Fluorescence-Assisted Synchrotron Infrared Microspectroscopy,Cell. Molec. Biol. 46 (2000), 1035-1044.

  11. Miller, L.M., Vairavamurthy, V., Chance, M.R., Paschalis, E.P., Betts, F., Boskey, A.L. and Mendelsohn, R.:In Situ Analysis of Mineral Crystallinity and Environment in Bone using Infrared Microspectroscopy,Biochim. Biophys. Acta 1527 (2000).

  12. Huang, R., Miller, L.M., Carlson, C.S. and Chance, M.R.: FTIR Analysis of Tibia Bone From Ovariectomized Cynomolgus Monkeys (Macaca fascicalaris) and the Effect of Nandrolone Decanoate Treatment,Bone 30 (2002), in press.

  13. Ali, S.R., Johnson, F.B., Luke, J.L. and Kalasinsky, V.F.: Characterization of Silicone Breast Inplant Biopsies by Fourier Transform Infrared Mapping,Cell. Mol. Biol. (Noisy-legrand) 44 (1998), 75-80.

  14. Kalasinsky, K.S., Magluilo, J., Jr. and Schaefer, T.: Hair Analysis by Infrared Microscopy for Drugs of Abuse,Forensic Sci. Int. 63 (1993), 253-260.

  15. Kalasinsky, K.S., Magluilo, J., Jr. and Schaefer, T.: Study of Drug Distribution in Hair by Infrared Microscopy Visualization,J. Anal. Toxicol. 18 (1994), 337-341.

  16. Kalasinsky, K.S.: Drug Distribution in Human Hair by Infrared Microscopy,Cell. Mol. Biol. (Noisy-le-grand) 44 (1998), 81-87.

  17. Carr, G.L., Reffner, J.A. and Williams, G.P.: Performance of an Infrared Microspectrometer at the NSLS,Rev. Sci. Instr. 66 (1995), 1490-1492.

  18. Reffner, J.A., Martoglio, P.A. and Williams, G.P.: Fourier Transform Infrared Microscopical Analysis with Synchrotron Radiation: The Microscope Optics and System Performance,Rev. Sci. Instr. 66 (1995), 1298.

  19. Carr, G.L. and Williams, G.P.: Infrared Microspectroscopy with Synchrotron Radiation,SPIE Conf. Proc. 3153 (1997), 51-59.

  20. Carr, G.L.: High-resolution Microspectroscopy and Sub-nanosecond Time-resolved Spectroscopy with the Synchrotron Infrared Source,Vibrational Spectroscopy 19 (1999), 53-60.

  21. Jamin, N., Dumas, P., Moncuit, J., Fridman, W.H., Teillaud, J.L., Carr, G.L. and Williams, G.P.: Chemical Imaging of Nucleic Acids, Proteins and Lipids of a Single Living Cell. Application of Synchrotron Infrared Microspectrometry in Cell Biology,Cell. Mol. Biol. (Noisy-le-grand) 44 (1998), 9-13.

  22. Jamin, N., Dumas, P., Moncuit, J., Fridman, W.H., Teillaud, J.L., Carr, G.L. and Williams, G.P.: Highly Resolved Chemical Imaging of Living Cells by using Synchrotron Infrared Microspectrometry,Proc. Natl. Acad. Sci. U.S.A. 95 (1998), 4837-4840.

  23. Holman, H.Y., Martin, M.C., Blakely, E.A., Bjornstad, K. and McKinney, W.R.: IR Spectroscopic Characteristics of Cell Cycle and Cell Death probed by Synchrotron Radiation based Fourier Transform IR Spectromicroscopy,Biopolymers 57 (2000), 329-335.

  24. Martin, M.C., Tsvetkova, N.M., Crowe, J.H. and McKinney, W.R.: Negligible Sample Heating from Synchrotron Infrared Beam,Appl. Spectr. 55 (2001), 111-113.

  25. Holman, H.Y., Bjornstad, K.A., McNamara, M.P., Martin, M.C., McKinney, W.R. and Blakely, E.A.: Synchrotron Infrared Spectromicroscopy as a Novel Bioanalytical Microprobe for Individual Living Cells: Cytotoxicity Considerations,J. Biomed. Opt. 7 (2002), 417-424.

  26. Tague, T. and Miller, L.M.: Novel Use of Fluorescence Illumination with an Infrared Microscope,Microscopy Today 2 (2000).

  27. Miller, L.M. and Tague, T.J.: Development and Biomedical Applications of Fluorescence-assisted Synchrotron Infrared Micro-spectroscopy,Vibrational Spectroscopy 849 (2002), 1-7.

  28. Miller, L.M., Dumas, P., Jamin, N., Teillaud, J.-L., Miklossy, J. and Forro, L.: Combining IR Spectroscopy and Fluorescence Imaging in a Single Microscope: Biomedical Applications using a Synchrotron Infrared Source,Rev. Sci. Instr. 73 (2002), 1357-1360.

  29. Xie, A., He, Q., Miller, L.M., Sclavi, B. and Chance, M.R.: Low Frequency Vibrations of Amino Acid Homo-Polymers observed by Synchrotron Far Infrared Absorption Spectroscopy: Excited State Effects dominate the Temperature Dependence of the Spectra,Biopolymers 49 (1999), 591-603.

  30. Wittlin, A., Genzel, L., Kremer, F., Häseler, S., Poglitsch, A. and Rupprecht, A.: Far-infrared Spectroscopy on Oriented Films of Dry and Hydrated DNA,Phys. Rev. A 34 (1986), 493-500.

  31. Markelz, A.G., Roitberg, A. and Heilweil, E.J.: Pulsed Terahertz Spectroscopy of DNA, Bovine Serum Albumin and Collagen between 0.1 and 2.0 THz,Chem. Phys. Lett. 320 (2000), 42-48.

  32. Globus, T.R., Woolard, D.L., Samuels, A.C., Gelmont, B.L., Hesler, J., Crowe, T.W. and Bykhovskaia, M.: Submillimeter-wave Fourier transform Spectroscopy of Biological Macromolecules,J. Appl. Phys. 91 (2002), 6105-6115.

  33. Marinkovic, N.S., Adzic, A.R., Sullivan, M., Kovacs, K., Miller, L.M., Rousseau, D.L., Yeh, S.R. and Chance, M.R.: Design and Implementation of a Rapid-Mix Flow Cell for Time-resolved Infrared Microspectroscopy,Rev. Sci. Instr. 71 (2000), 4057-4060.

  34. Miller, L.M., Sullivan, M., Toomey, J., Marinkovic, N., Carr, G.L., Williams, G.P. and Chance, M.R.: Biological InfraredMicrospectroscopy at the National Synchrotron Light Source,Synchr. Rad. Instr. 521 (1999), 47-50.

  35. Plunkett, S.E., Jonas, R.E. and Braiman, M.S.: Vibrational Spectra of Individual Millimetersize Membrane Patches using Miniature Infrared Waveguides,Biophys. J. 73 (1997), 2235-2240.

  36. Palanker, D.V., Knippels, G.H., Smith, T.I. and Swettman, H.A.: IR Microscopy with a Transient Photo-Induced Near-Field Probe (Tipless Near-Field Microscopy),Optics Commun. 148 (1998), 215-220.

  37. Knoll, B. and Keilmann, F.: Near-field Probing of Vibrational Absorption for Chemical Microscopy,Nature 399 (1999), 134.

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Authors and Affiliations

  1. Brookhaven National Laboratory, National Synchrotron Light Source, Upton, NY, 11973, USA

    L.M. Miller, G.D. Smith & G.L. Carr

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  1. L.M. Miller
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  2. G.D. Smith
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  3. G.L. Carr
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Miller, L., Smith, G. & Carr, G. Synchrotron-based Biological Microspectroscopy: From the Mid-Infrared through the Far-Infrared Regimes. Journal of Biological Physics 29, 219–230 (2003). https://doi.org/10.1023/A:1024401027599

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