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Alveolar Epithelial Cell-Macrophage Interactions Affect Oxygen-Stimulated Interleukin-8 Release

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Abstract

Interactions of alveolar macrophages with respiratory epithelium may play a key role in hyperoxia-induced lung inflammation. We studied the effect of cell–cell contact with epithelial cells in hyperoxia on macrophages' secretion of interleukin-8 (IL-8). A549 pulmonary epithelial cells and THP-1 monocyte/macrophage cells were cultured either singly, in contact coculture, or prevented from contact by a porous membrane, and exposed to oxygen or room air. Phorbol-12-myristate-13-acetate-(PMA)-treated THP-1 cells were exposed to the same conditions. Neither cell line cultured alone produced appreciable amounts of IL-8 in hyperoxia. Contact cocultures exposed to hyperoxia produced increased IL-8, while in the noncontact coculture it was attenuated. Both cell–cell contact and PMA increased THP-1 cell CD54 expression. Intracellular IL-8 production was increased in contact cocultured, hyperoxia exposed THP-1 cells, while the A549 sample showed no change. Increased IL-8 mRNA expression was not demonstrated in cocultured, hyperoxic exposed THP-1 cells, suggesting nontranscriptional regulation of IL-8 protein levels. Contact with epithelial cells appears to potentiate macrophage responses to hyperoxia.

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Authors and Affiliations

  1. Division of Neonatology, Strong Children's Research Center, University of Rochester, Rochester, New York

    Matthew R. Hjort, Andrew J. Brenyo, Jacob N. Finkelstein, Michael B. LoMonaco, Carl J. Johnston & Carl T. D'Angio

  2. Pulmonary and Critical Care Division, University of Rochester, Rochester, New York

    Mark W. Frampton & Judith C. Stewart

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  1. Matthew R. Hjort
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  2. Andrew J. Brenyo
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  3. Jacob N. Finkelstein
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  4. Mark W. Frampton
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  5. Michael B. LoMonaco
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  8. Carl T. D'Angio
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Hjort, M.R., Brenyo, A.J., Finkelstein, J.N. et al. Alveolar Epithelial Cell-Macrophage Interactions Affect Oxygen-Stimulated Interleukin-8 Release. Inflammation 27, 137–145 (2003). https://doi.org/10.1023/A:1023817811850

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