Abstract
A plasmid pSDK-1 containing the Escherichia coli phosphofructokinase-1 gene (pfkA) was constructed, and transferred into Acidithiobacillus thiooxidans Tt-7 by conjugation. The pfkA gene from E. coli could be expressed in this obligately autotrophic bacterium but the enzyme activity (18 U g−1) was lower than that in E. coli (K12: 86 U g−1; DF1010 carrying plasmid pSDK-1: 97 U g−1). In the presence of glucose, the Tt-7 transconjugant consumed glucose leading to a better growth yield.
Similar content being viewed by others
References
Berger DK, Woods DK, Rawlings DE (1990) Complementation of Escherichia coli ? 54 (ntrA)-dependent formatehydrogen-lyase activity by a cloned T. ferrooxidans ntrA gene. J. Bacteriol. 172: 4399–4406.
Darbre A (1986) Analytical methods. In: Practical Protein Chemistry: A Handbook. New York: John Wiley and Sons, pp. 227–335.
Davison J, Heusterspreute M, Chevalier N, Ha ThiV, Brunel F (1987) Vectors with restriction site banks V. pJRD215, a widehost-range cosmid vector with multiple cloning sites. Gene 51: 275–280.
Guiliani N, Bengrine A, Borne F, Chippaux, Bonnefoy V (1997) Alanyl-tRNA synthetase gene of the extreme acidophilic chemolithoautotrophic Thiobacillus ferrooxidans is highly homologous to alaS genes from all living kingdoms but cannot be transcribed from its promoter in Escherichia coli. Microbiology 143: 2179–2187.
Inoue H, Tamura T, Ehara N, Nishito A, Nakayama Y, Maekawa M, Imada K, Tanaka H, Inagaki K (2002) Biochemical and molecular characterization of the NAD+-dependent isocitrate dehydrogenase from the chemolithotroph Acidithiobacillus thiooxidans. FEMS Microbiol. Lett. 214: 127–132.
Jin SM, Yan WM, Wang ZN (1992) Transfer of IncP plasmids to extremely acidophilic Thiobacillus thiooxidans. Appl. Environ. Microbiol. 58: 429–430.
Jin SM, Yan WM, Wang ZN (1993) Development of a conjugative transfer system for Thiobacillus thiooxidans. Chinese J. Biotechnol. 9: 87–89.
Kotlarz D, Buc H (1982) Phosphofructokinases from Escherichia coli. Meth. Enzymol. 90: 60–70.
Kusano T, Sugawara K, Inoue C, Suzuki N (1991) Molecular cloning and expression of Thiobacillus ferrooxidans chromosomal ribulose bisphosphate carboxylase genes in Escherichia coli. Curr. Microbiol. 22: 35–41.
Matin A (1978) Organic nutrition of chemolithotrophic bacteria. Annu. Rev. Microbiol. 32: 433–468.
Shao Q, Yan WM, Liu ZHY, Yao DY (1997) Construction of arsenic resistance gene containing plasmid pSGR941 and its expression in the obligately autotrophic acidophilic Thiobacillus thiooxidans. J. Shandong University (China) 32: 348–352.
Simon R, Priefer U, Puhier A (1983) A broad host range mobilization system for in vitro genetic engineering: transposon mutagenesis in gram negative bacteria. Bio/Technology 1: 784–791.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Tian, K.L., Lin, J.Q., Liu, X.M. et al. Conversion of an obligate autotrophic bacteria to heterotrophic growth: expression of a heterogeneous phosphofructokinase gene in the chemolithotroph Acidithiobacillus thiooxidans . Biotechnology Letters 25, 749–754 (2003). https://doi.org/10.1023/A:1023588921918
Issue Date:
DOI: https://doi.org/10.1023/A:1023588921918