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Induced reactive oxygen species improve enzyme production from Aspergillus niger cultivation

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Abstract

Intracellular reactive oxygen species (iROS) induction by HOCl was used as a novel strategy to improve enzyme productivities in Aspergillus niger growing in a bioreactor. With induced iROS, the specific intracellular activities of α-amylase, protease, catalase, and glucose oxidase were increased by about 170%, 250%, 320%, and 260%, respectively. The optimum specific iROS level for achieving maximum cell concentration and enzyme production was about 15 mmol g cell−1. The type of iROS inducing the enzyme production was identified to be a derivative of the superoxide radical.

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Authors and Affiliations

  1. Biochemical Engineering Group, Department of Chemical Engineering, Indian Institute of Technology, Bombay, Powai, Mumbai, 400 076, India

    Susmita Sahoo & G.K. Suraishkumar

  2. School of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Powai, Mumbai, 400 076, India

    K. Krishnamurthy Rao

Authors
  1. Susmita Sahoo
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  2. K. Krishnamurthy Rao
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  3. G.K. Suraishkumar
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Correspondence to G.K. Suraishkumar.

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Sahoo, S., Krishnamurthy Rao, K. & Suraishkumar, G. Induced reactive oxygen species improve enzyme production from Aspergillus niger cultivation. Biotechnology Letters 25, 821–825 (2003). https://doi.org/10.1023/A:1023508609623

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  • DOI: https://doi.org/10.1023/A:1023508609623

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