Abstract
Bioenergetics of tomato (Lycopersicon esculentum) development on the plant was followed from the early growing stage to senescence in wild type (climacteric) and nonripening mutant (nor, nonclimacteric) fruits. Fruit development was expressed in terms of evolution of chlorophyll a content allowing the assessment of a continuous time-course in both cultivars. Measured parameters: the cytochrome pathway-dependent respiration, i.e., the ATP synthesis-sustained respiration (energy-conserving), the uncoupling protein (UCP) activity-sustained respiration (energy-dissipating), the alternative oxidase(AOX)-mediated respiration (energy-dissipating), as well as the protein expression of UCP and AOX, and free fatty acid content exhibited different evolution patterns in the wild type and nor mutant that can be attributed to their climacteric/nonclimacteric properties, respectively. In the wild type, the climacteric respiratory burst observed in vitro depended totally on an increse in the cytochrome pathway activity sustained by ATP synthesis, while the second respiratory rise during the ripening stage was linked to a strong increase in AOX activity accompanied by an overexpression of AOX protein. In wild type mitochondria, the 10-μM linoleic acid-stimulated UCP-activity-dependent respiration remained constant during the whole fruit development except in senescence where general respiratory decay was observed.
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REFERENCES
Almeida, A. M., Jarmuszkiewicz, W., Khomsi, H., Arruda, P., Vercesi, A. E., and Sluse, F. E. (1999). Plant Physiol. 119, 1323–1329.
Andreyev, A. Y., Bondareva, T. O., Dedukhova, V. I., Mokhova, E. N., Skulachev, V. P., Tsofina, L. M., Volkov, N. L., and Vygodina, T. V. (1989), Eur. J. Biochem. 182, 585–592.
Beadle, N. C. W. (1937). Aust. J. Exp. Biol. Med. Sci. 15, 173–189.
Bergevin, M., L'Heureux, G. P., Thompson, J. E., and Willemot, C. (1993). Physiol. Plant. 87, 522–527.
Brady. C. J. (1987). Annu. Rev. Plant Physiol. 38, 155–178.
Considine, M., Daley, D., and Whelan, J. (2001). Plant Physiol. 126, 1619–1629.
Deby-Dupont, G., Ducarne, H., de Lanndsheere, C., Ancion, J. C., Noel, F. X., Dadoux, L., and Deby, C. (1983). Biomed. Pharmacother. 37, 386–391.
Ferguson, I., Volz, R., and Woolf, A. (1999). Postharv. Biol. Tech. 15, 255–262.
Folch, J., Lees, M., and Sloane-Stanley, G. H. (1957). J. Biol. Chem. 226, 497–509.
Gornall, A. G., Bardawill, C. J., and Dawid, M. M. (1949). J. Biol. Chem. 177, 751–757.
Jarmuszkiewicz, W., Almeida, A. M., Sluse-Goffart, C. M., Sluse, F. E., and Vercesi, A. E. (1998). J. Biol. Chem. 273, 34882–34886.
Jarmuszkiewicz W., Almeida, A. M., Sluse-Goffart, C. M., Sluse, F. E., and Vercesi, A. E. (2000). J. Biol. Chem. 275, 13315–13320.
Jezek, P., Costa, A. D. T., and Vercesi, A. E. (1996). J. Biol. Chem. 271, 32743–32748.
Jezek, P., Costa, A. D. T., and Vercesi, A. E. (1997). J. Biol. Chem. 272, 24272–24278.
Klingenberg, M., and Echtay, K. S. (2001). Biochim. Biophys. Acta 1504, 128–143.
Klingenberg, M., and Huang, S. G. (1999). Biochim. Biophys. Acta 1415, 271–296.
Kumar, S., and Sinha, S. H. (1992). J. Exp. Bot. 43, 1639–1642.
Kumar, S., Patil, B. C., and Sinha, S. K. (1990). Biochem. Biophys. Res. Commun. 168, 818–822.
Lelievre, J.-M., Latche, A., Jones, B., Bouzayen, M., and Pech, J.-C. (1997). Physiol. Plant. 101, 727–739.
Lyons, J., and Pratt, H. K. (1963). Am. Soc. Hort. Sci. 84, 491–500.
Meeuse, B. J. D. (1975). Annu. Rev. Plant Physiol. 26, 117–126.
Minagawa, N., Sakajo, S., and Yoshimoto, A. (1992). Biosci. Biotech. Biochem. 56, 1342–1343.
Moller, I. M., and Palmer, J. M. (1982). Physiol. Plant. 54, 267–274.
Pirrung, M. C., and Brauman, J. I. (1987). Plant. Physiol. Biochem. 25, 55–61.
Schlenk, H. L., and Gellerman, J. L. (1960), Anal. Chem. 32, 1412–1414.
Sluse, F. E., Almeida, A. M., Jarmuszkiewicz, W., and Vercesi, A. E. (1998). FEBS Lett. 433, 237–240.
Sluse, F. E., and Jarmuszkiewicz, W. (1998). Braz. J. Med. Biol. Res. 32, 733–747.
Sluse, F. E., and Jarmuszkiewicz, W. (2000). Braz. J. Med. Biol. Res. 33, 259–268.
Sluse, F. E., and Jarmuszkiewicz, W. (2002). FEBS Lett. 510, 117–120.
Solomos, T (1977). Annu. Rev. Plant Physiol. 28, 279–297.
Szarkowska, L., and Klingenberg, M. (1963). Biochem. Z. 338, 674–697.
Tigchelaar, E. C., McGlasson, W. B., and Buescher, R.W. (1978). Hort. Sci. 13, 508–513.
Tucker, M. L., and Laties, G. G. (1985). Plant Cell. Environ. 8, 117–127.
Umbach, A. L., and Siedow, J. N. (1993). Plant Physiol. 103, 845–854.
Vanlerbergue, G. C., and McIntosh, L. (1997). Annu. Rev. Plant Physiol. Plant Mol. Biol. 48, 703–734.
Vercesi, A. E., Martins, I. S., Silva, M. A. P., Leite, H. M. F., Cucovia, I. M., and Chaimovich, H. (1995). Nature 375, 24.
Wagner, A. M., and Moore, A. L. (1997). Biosci. Rep. 17, 319–333.
Wieckowski, M., and Wojtczak, L. (1997). Biochem. Biophys. Res. Commun. 232, 414–417.
Woolf, A. B., Wexler, A., Prusky, D. N., Kobiler, E., and Lurie, S. (2000). J. Am. Soc. Hort. Sci. 125, 370–376.
Zackova, M., Kramer, R., and Jezek, P. (2000). Int. J. Biochem. Cell Biol. 32, 499–508.
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Almeida, A.M., Navet, R., Jarmuszkiewicz, W. et al. The Energy-Conserving and Energy-Dissipating Processes in Mitochondria Isolated from Wild Type and Nonripening Tomato Fruits During Development on the Plant. J Bioenerg Biomembr 34, 487–498 (2002). https://doi.org/10.1023/A:1022574327117
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DOI: https://doi.org/10.1023/A:1022574327117