Skip to main content
SpringerLink
Log in

Steady-State Proton Translocation in Bovine Heart Mitochondrial bc 1 Complex Reconstituted into Liposomes

  • Published:
Journal of Bioenergetics and Biomembranes Aims and scope Submit manuscript

Abstract

The effect of different anions on the steady-state proton translocation in bovine bc 1 complex reconstituted in liposomes was studied. The H+/e − ratio for vectorial proton translocation is at the steady state definitely lower than that measured at level flow, (0.3 vs. 1.0). The presence of azide or arachidonate at micro- and submicromolar concentrations, respectively, gave a substantial reactivation of the proton pumping activity at the steady state, without any appreciable effect on respiration-dependent transmembrane pH difference. Addition of azide to turning-over bc 1 vesicles also caused a transition of b cytochromes toward oxidation. The results are discussed in terms of possible involvement of an acidic residue in the protonation of the semiquinone/quinol couple at the N side of the membrane.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  • Beattie, D. S. (1993). J. Bioenerg. Biomembr. 25, 233–244.

    Google Scholar 

  • Borchart, U., Machleidt, W., Schagger, H., Link, T. A., and von Jagow, G. (1985). FEBS Lett. 191, 125–130.

    Google Scholar 

  • Braiman, M. S., Mogi, T., Stern L. J., Hackett, N., Chao, B. H., Khorana, H. G., and Rothschild, K. J. (1988). Proteins: Struct. Fund. Genet. 3, 219–229.

    Google Scholar 

  • Brand, M. D., Chien, L., and Diolez P. (1994). Biochem. J. 297, 27–29.

    Google Scholar 

  • Brasseur, R. (1988). J. Biol. Chem. 263, 12571–12575.

    Google Scholar 

  • Brown, G. C. (1989). J. Biol. Chem. 264, 14704–14709.

    Google Scholar 

  • Bruel, C., Manon, S., Guérin, M., and Lemesle-Meunier, D. (1995). J. Bioenerg. Biomembr. 27, 527–539.

    Google Scholar 

  • Canton, M., Luvisetto, S., Schmehl, I., and Azzone, G. F. (1995). Biochem. J. 310, 477–481.

    Google Scholar 

  • Clejan, L., Bosch, L. G., and Beattie, D. S. (1984). J. Biol. Chem. 259, 11169–11172.

    Google Scholar 

  • Cocco, T., Lorusso, M., Sardanelli, A. M., Minuto, M., Ronchi, S., Tedeschi, G., and Papa, S. (1991). Eur. J. Biochem. 195, 731–734.

    Google Scholar 

  • Cocco, T., Lorusso, M., Di Paola, M., Minuto, M., and Papa, S. (1992). Eur. J. Biochem. 209, 475–481.

    Google Scholar 

  • Crofts, A. R., Robinson, H., Andrews, K., Van Doren, S. R., and Berry, E. (1987). In Cytochrome Systems: Molecular Biology and Bioenergetics (Papa, S., Chance, B., and Ernster, L., eds.), Plenum Press, New York, pp. 617–624.

    Google Scholar 

  • Degli Esposti, M., Saus, J., Timoneda, J., Bertoli, E., and Lenaz, G. (1982). FEBS Lett. 147, 101–105.

    Google Scholar 

  • Degli Esposti, M., Meier, E. M. M., Timoneda, J., and Lenaz, G. (1983). Biochim. Biophys. Acta 725, 349–390.

    Google Scholar 

  • Degli Esposti, M., De Vries, S., Crimi, M., Ghelli, A., Patarnello, T., and Meyer, A. (1993). Biochim. Biophys. Acta 1143, 243–271.

    Google Scholar 

  • Errede, B., Kamen, M. O., and Hatefi, Y. (1978). Methods Enzymol. 52, 40–47.

    Google Scholar 

  • Gennis, R. B., Barquera, B., Hacker, B., Van Doren, S. R., Arnaud, S., Crofts, A. R., Davidson, E., Gray, K. A., and Daldal, F. (1993). J. Bioenerg. Biomembr. 25, 195–209.

    Google Scholar 

  • Gerwert, K., Hess, B., Soppa, J., and Oesterhelt, D. (1989). Proc. Natl. Acad. Sci. USA 86, 4943–4947.

    Google Scholar 

  • Hafner, R. P., and Brand, M. D. (1991). Biochem. J. 275, 75–80.

    Google Scholar 

  • Hassinen, I. E., and Vuokila P. T. (1993). Biochim. Biophys. Acta 1144, 107–124

    Google Scholar 

  • Konstantinov, A. A., and Popova, E. (1987). In Cytochrome System: Molecular Biology and Bioenergetics (Papa, S., Chance, B., and Ernster, L., eds.), Plenum Press, New York, pp. 751–765.

    Google Scholar 

  • Leung, K. H., and Hinkle, P. C. (1975). J. Biol. Chem. 250, 8467–8471.

    Google Scholar 

  • Lorusso, M., Gatti, D., Boffoli, D., Bellomo, E., and Papa, S. (1983). Eur. J. Biochem. 137, 413–420.

    Google Scholar 

  • Lorusso, M., Cocco, T., Boffoli, D., Gatti, D., Meinhardt, S. W., Ohnishi, T., and Papa, S. (1989). Eur. J. Biochem. 179, 535–540.

    Google Scholar 

  • Lorusso, M., Cocco, T., Minuto, M., Capitanio, N., and Papa, S. (1995). J. Bioenerg. Biomembr. 27, 101–108.

    Google Scholar 

  • Luvisetto, S., Conti, E., Buso, M., and Azzone, G. F. (1991). J. Biol. Chem. 266, 1034–1042.

    Google Scholar 

  • Murphy, M. P., and Brand, M. D. (1988). Eur. J. Biochem. 173, 645–651.

    Google Scholar 

  • Nalecz, M. J., Casey, R. P., and Azzi, A. (1983). Biochim. Biophys. Acta 724, 75–82.

    Google Scholar 

  • Ohnishi, T., Schagger, H., Meinhardt, S. W., Lo Brutto, R., Link, T. A., and von Jagow, G. (1989). J. Biol. Chem. 264, 735–744.

    Google Scholar 

  • Paddock, M. L., Rongey, S. H., Mc Pherson, P. H., Juth, A., Feher, G., and Okamura, M. Y. (1994). Biochemistry 33, 734–745.

    Google Scholar 

  • Palmer, T., Williams, R., Cotton, N. P., Thomas, C. M., and Jackson, J. B. (1993). Eur. J. Biochem. 211, 663–669.

    Google Scholar 

  • Papa, S., Guerrieri, F., Lorusso, M., Izzo, G., Boffoli, D., Capuano, F., Capitanio, N., and Altamura, N. (1980). Biochem. J. 192, 203–218.

    Google Scholar 

  • Papa, S., Lorusso, M., Izzo, G., and Capuano, F. (1981). Biochem. J. 194, 395–406.

    Google Scholar 

  • Papa, S., Lorusso, M., Boffoli, D., and Bellomo, E. (1983). Eur. J. Biochem. 137, 405–412.

    Google Scholar 

  • Papa, S., Lorusso, M., and Capitanio, N. (1994). J. Bioenerg. Biomembr. 26, 609–618.

    Google Scholar 

  • Rao, J. K., and Argos, P. (1986). Biochim. Biophys. Acta 869, 197–205.

    Google Scholar 

  • Rich, P. R., and Heathcote, P. (1983). Sixth International Congress on Photosynthesis, Brussels, Abstracts, pp. 363–366.

  • Rieske, J. S. (1967). Methods Enzymol. 10, 239–245.

    Google Scholar 

  • Takahashi, E., and Wraight, C. A. (1991). FEBS Lett. 283, 140–144.

    Google Scholar 

  • Tittor, J., Soell, C., Oesterhelt, D., Butt, H., and Bamberg E. (1989). EMBO J. 8, 3477–3482.

    Google Scholar 

  • von Jagow, G., and Engel, W. D. (1981). FEBS Lett. 136, 19–24.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Medical Biochemistry and Chemistry, University of Bari, Italy

    Tiziana Cocco, Marco Di Paola, Michele Minuto, Valeria Carlino, Sergio Papa & Michele Lorusso

Authors
  1. Tiziana Cocco
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Marco Di Paola
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Michele Minuto
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Valeria Carlino
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sergio Papa
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Michele Lorusso
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cocco, T., Paola, M.D., Minuto, M. et al. Steady-State Proton Translocation in Bovine Heart Mitochondrial bc 1 Complex Reconstituted into Liposomes. J Bioenerg Biomembr 29, 81–87 (1997). https://doi.org/10.1023/A:1022467923837

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1022467923837

Search

Navigation