Skip to main content
SpringerLink
Log in

Neutrophil Behavior Following Exposure to in Vivo or in Vitro Zinc in Normal and Acutely-Inflamed Rats: Studies on Lysozyme Secretion, Superoxide Anion Release and Platelet Adhesion

  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

The mechanism was studied of the anti-inflammatory effect of oral zinc (114 mg/kg/day of elemental metal, given for 14 days) on the development of the carrageenan-induced paw oedema of the rat, and the impact of in vivo treatment on the activity of neutrophils isolated from the blood of inflamed and non-inflamed animals. The effects of the in vitro incubation with the metal on either non-inflamed or inflamed neutrophils coming from zinc-untreated rats were also examined. It was found that the administration of oral zinc inhibited markedly the process of ex vivo adhesion of the cells obtained from the inflamed rats (an observation confirmed by the in vitro experiments). In vitro release of lysozyme and superoxide anion productions were measured: in the absence of zinc, the 30′ of pre-incubation carried out before stimulating with PMA did not influence the cell's reactivity of the non-inflamed neutrophils. It was, on the contrary, capable of significantly reducing that of the inflamed ones. As a consequence, it is quite difficult to properly interpret the data obtained studying the activity of the cells exposed to the metal in vitro.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Denko, C. W., M. Petricevic, and M. W. Whitehouse. 1981. Inflammation in relation to dietary intake of zinc and copper. Int. J. Tiss. Reac. 3:73–76.

    Google Scholar 

  2. Milanino, R., G. P. Velo, and M. Marrella. 1996. Copper and zinc in the pathophysiology and treatment of inflammatory disorders. In: Therapeutic uses of trace elements. Neve J., P. Chappuis and M. Lamand, editors. New York: Plenum Publishing Corporation, 115–126.

    Google Scholar 

  3. Milanino, R., M. Marrella, U. Moretti, E. Concari, and G. P. Velo. 1988. Copper and zinc status in rats with acute inflammation: focus on the inflamed area. Agents and Actions 24:356–364.

    Google Scholar 

  4. Milanino, R., U. Moretti, E. Concari, M. Marrella, and G. P. Velo. 1988. Copper and zinc status in adjuvant arthritic rat: studies on blood, liver, kidneys, spleen and inflamed paws. Agents and Actions 24:365–376.

    Google Scholar 

  5. Milanino, R., M. Marrella, R. Gasperini, M. Pasqualicchio, and G. P. Velo. 1993. Copper and zinc body levels in inflammation: an overview of the data obtained from animal and human studies. Agents and Actions 39:195–209.

    Google Scholar 

  6. Pekarek, R. S. and G. W. Evans. 1975. Effect of acute infection and endotoxemia on zinc absorption in the rat. Proc. Soc. Exp. Biol. Med. 150:755–758.

    Google Scholar 

  7. Cousins, R. J. and M. R. Swerdel. 1985. Ceruloplasmin and metallothionein induction by zinc and 13-cisretinoic acid in rats with adjvant inflammation. Proc. Soc. Exp. Biol. Med. 179:168–172.

    Google Scholar 

  8. Abou-Mohamed, G., H. A. El-Kashef, H. A. Salem, and M. M. Elmazar. 1995. Effect of zinc on the anti-inflammatory and ulcerogenic activities of indomethacin and diclofenac. Pharmacology 50:266–272.

    Google Scholar 

  9. Whitehouse, M. W., K. D. Rainsford, R. M. Taylor, and B. Vernon-Roberts. 1990. Zinc monoglycerolate: a slow-release source of zinc with anti-arthritic activity in rats. Agents and Actions 31:47–58.

    Google Scholar 

  10. Nagai, H., K. Kitagaki, K. Kuwabara, and A. Koda. 1992. Anti-inflammatory properties of zinc protoporphyrin disodium (Zn-PP-2Na). Agents and Actions 37:273–283.

    Google Scholar 

  11. Marrella, M., R. Gasperini, E. Rizzarelli, G. P. Velo, and R. Milanino. 1994. Repeated oral zinc administration in the rat: anti-inflammatory effect and changes of zinc and copper body status in inflamed male and female animals. In: Metal Ions in Biology and Medicine, Vol. 4. Collery Ph., L. A. Poirier, N. A. Littlefield, and J. C. Etienne, editors. John Libbey Eurotext, Paris 571–575.

    Google Scholar 

  12. Simkin, P. A. 1976. Oral zinc sulphate in reumatoid arthritis. Lancet II:539–542.

    Google Scholar 

  13. Frigo, A., L. M. Bambara, E. Concari, M. Marrella, U. Moretti, C. Tambalo, G. P. Velo, and R. Milanino. 1989. Concerning the potential therapeutic value of zinc in rheumatoid and psoriatic arthritis. In: Copper and zinc in inflammation. R. Milanino, G. P. Velo and K. D. Rainsford, editors. Dordrecht: Kluwer, 133–142.

    Google Scholar 

  14. Albergoni, V. Physiological properties of copper and zinc. In: Copper and zinc in inflammatory and degenerative diseases. K. D. Rainsford, R. Milanino, J. R. J. Sorenson and G. P. Velo, editors. Kluwer Academic Publisher (in press) Dordrecht.

  15. Prasad, A. S. 1983. The role of zinc in gastrointestinal and liver diseases. Clin. Gastroenterol. 12:713–741.

    Google Scholar 

  16. Milanino, R., U. Moretti, M. Marrella, M. Pasqualicchio, R. Gasperini, and G. P. Velo. 1995. Copper and zinc in the development and control of inflammation. In: Handbook on metal-ligand interactions in biological fluids, Vol. 2. G. Berthon, editor. Marcel Dekker Inc., New York: 886–899.

    Google Scholar 

  17. Chvapil, M., L. Stankova, C. Zukoski IV, and C. Zukoski III. 1977. Inhibition of some functions of polymorphonuclear leukocytes by in vitro zinc. J. Lab. Clin. Med. 89:135–146.

    Google Scholar 

  18. Yatsuyanagi, J., K. Iwai, and T. Ogiso. 1987. Suppressive effect of zinc on some functions of neutrophils: studies with carrageenan-induced inflammation in rats. Chem. Pharm. Bull. 35:699–704.

    Google Scholar 

  19. Cook-Mills, J. M., J. J. Wirth, and P. J. Fraker. 1990. Possible roles for zinc in destruction of Trypanosoma cruzi by toxic oxygen metabolites produced by mononuclear phagocytes. Adv. Exp. Med. Biol. 262:111–121.

    Google Scholar 

  20. Vruwink, K. G., M. P. Fletcher, C. L. Keen, M. S. Golub, A. G. Hencrickx, and M. E. Gershwin. 1991. Moderate zinc deficiency in rhesus monkeys: an intrinsic defect of neutrophil chemotaxis corrected by zinc repletion. J. Immunol. 146:244–249.

    Google Scholar 

  21. Beswick, P. H., P. C. Brannen, and S. S. Hurles. 1986. The effects of smoking and zinc on the oxidative reactions of human neutrophils. J. Clin. Lab. Immunol. 21:71–75.

    Google Scholar 

  22. Malamud, D., A. Scott, D. Shapiro, and I. M. Shapiro. 1985. Low levels of mercury inhibit the respiratory burst in human polymorphonuclear leukocytes. Biochem. Biophys. Res. Commun. 128:1145–1151.

    Google Scholar 

  23. Baginski, B. 1985. Einfluß von Blei und Cadmium auf die Vitalität und Phagozytosefähigkeit humaner polymorphkerniger Leukozyten. Zbl. Bakt. Hyg. I. Abt. Orig. B 181:461–468.

    Google Scholar 

  24. Marrella, M., and R. Milanino. 1986. Simple and reproducible method for extraction of copper and zinc from rat tissue for determination by flame atomic absorption spectroscopy. Atomic Spectrosc. 7:40–42.

    Google Scholar 

  25. Bellavite, P., S. Chirumbolo, C. Mansoldo, G. Gandini, and P. Dri. 1992. Simultaneous assay for oxidative metabolism and adhesion of human neutrophils: evidence for correlations and dissociations of the two responses. J. Leukocyte Biol. 51:329–335.

    Google Scholar 

  26. Cousins, R. J. 1985. Absorption, transport, and hepatic metabolism of copper and zinc: special reference to metallothionein and ceruloplasmin. Physiol. Rev. 65:238–309.

    Google Scholar 

  27. Brewer, G. J., G. M. Hill, A. S. Prasad, Z. T. Cossack, and P. Rabbani. 1983. Oral zinc therapy for Wilson's disease. Ann. Intern. Med. 99:314–320.

    Google Scholar 

  28. Milanino, R., A. Deganello, M. Marrella, F. Michielutti, U. Moretti, M. Pasqualicchio, G. Tamassia, L. TatÒ, and G. P. Velo. 1992. Oral zinc as initial therapy in Wilson's disease: two years of continuous treatment in a 10-year-old child. Acta Poediatr. 81:163–166.

    Google Scholar 

  29. Milanino, R., S. Mazzoli, E. Passarella, G. Tarter, and G. P. Velo. 1978. Carrageenan oedema in copper-deficient rats. Agents and Actions 8:618–622.

    Google Scholar 

  30. Milanino, R., A. Conforti, M. E., Fracasso, L. Franco, R. Leone, E. Passarella, G. Tarter, and G. P. Velo. 1979. Concerning the role of endogenous copper in the acute inflammatory process. Agents and Actions 9:581–588.

    Google Scholar 

  31. Denko, C. W. 1979. Protective role of ceruloplasmin in inflammation. Agents and Actions 9:333–336.

    Google Scholar 

  32. Pasqualicchio, M., M. E. Davies, and G. P. Velo. 1995. Effects of copper and zinc on chondrocyte-mononuclear cell adhesion via ICAM-1/CD18 interactions. Inflammopharmacology 3:35–48.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Istituto di Farmacologia, Facoltà di Medicina e Chirurgia, Università di Verona, Italy

    Giuseppina Benoni, Laura Cuzzolin, Mauro Marrella, Federica Crivellente, Alessandra Adami, Chiara Bertaso, Giampaolo Velo & Roberto Milanino

Authors
  1. Giuseppina Benoni
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Laura Cuzzolin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mauro Marrella
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Federica Crivellente
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Alessandra Adami
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Chiara Bertaso
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Giampaolo Velo
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Roberto Milanino
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Benoni, G., Cuzzolin, L., Marrella, M. et al. Neutrophil Behavior Following Exposure to in Vivo or in Vitro Zinc in Normal and Acutely-Inflamed Rats: Studies on Lysozyme Secretion, Superoxide Anion Release and Platelet Adhesion. Inflammation 22, 175–189 (1998). https://doi.org/10.1023/A:1022388022947

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1022388022947

Keywords

Search

Navigation