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Gastrin radioimmunoassay: Description and application of a novel method

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Abstract

In the present paper the development and application of a novel gastrin radioimmunoassay (RIA) are described. 125I-labeling of non-sulphated human gastrin-17 (nshG-17) was performed by the iodogen method and the mono-iodinated hormone, as RIA tracer, was separated by reversed-phase high performance liquid chromatography (HPLC). Serum gastrin levels were measured in response to intravenous application of isoproterenol, a non-selective beta and phenylephrine, a selective alpha-1 receptor agonist using a newly developed method specific for the C-terminal part of the hormone in rats. Isoproterenol at clinically relevant doses elicited a significant increase in serum gastrin concentration in a dose-dependent fashion, whereas phenylephrine was without effect.

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Authors and Affiliations

  1. Neuropharmacology Research Group of the Hungarian Academy of Sciences, Department of Pharmacology and Pharmacotherapy, University of Pécs, H-7643, P. O. Box 99, Pécs, Hungary

    J. Németh, B. Jakab, I. Schweibert & J. Szolcsányi

  2. Department of Pharmacology, University of Debrecen, H-4029, Nagyerdei krt 98, Debrecen, Hungary

    G. Oroszi & Z. Szilvássy

Authors
  1. J. Németh
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  2. G. Oroszi
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  3. B. Jakab
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  4. I. Schweibert
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  5. J. Szolcsányi
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  6. Z. Szilvássy
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Németh, J., Oroszi, G., Jakab, B. et al. Gastrin radioimmunoassay: Description and application of a novel method. Journal of Radioanalytical and Nuclear Chemistry 254, 277–281 (2002). https://doi.org/10.1023/A:1021663514566

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