Abstract
The aim of this study was to determine the relationship between changes in microporosity and the osseointegration and the osseoconductivity of hydroxyapatite (HAp). HAp cylinders were manufactured by a combination of uniaxial powder pressing and different sintering conditions, with different percentages of microporosity: HAp-I with 3.96±0.75% microporosity, HAp-II with 15.66±1.60% microporosity, and HAp-III with 29.72±0.69% microporosity. These HAp cylinders were surgically implanted in rabbit femurs. After 8 and 12 weeks, the femurs were removed, fixed, sectioned, ground, and stained by Stevenel's blue/Van Gieson for light microscopy and histomorphometry. Some ground sections were routinely processed for SEM. The osseointegration and the osseoconductivity were determined by means of image analysis and the data were submitted to ANOVA. In all cases the cortical bone was repaired and the HAp facing the medullary canal was lined with endosteum, which in some areas exhibited thin bone tissue formation. SEM observations showed no differences in the morphology of tissue-HAp interfaces for the three different porosities of HAp. There were no statistical differences between the groups related to either osseointegration or osseoconductivity. These results suggest that neither osseointegration nor osseoconductivity of HAp are influenced by changes in HAp microporosity.
Similar content being viewed by others
References
P. S. Eggli, W. Muller and R. K. Schenk, Clin. Orthop. 232 (1988) 127.
A. L. Rosa, J. A. Devlin, P. V. Hatton and R. Van Noort, J. Dent. Res. 78 (1999) 409.
F. R. Wang, Nippon Seikeigeka Gakkai Zasshi 64 (1990) 847.
H. Schliephake, F. W. Neukam and D. Klosa, Int. J. Oral Maxillofac. Surg. 20 (1991) 53.
J. H. Kuhne, R. Barti, B. Frisch, C. Hammer, V. Jansson and M. Zimmer, Acta Orthop. Scand. 65 (1994) 246.
E. Tsuruga, H. Takita, H. Itoh, Y. Wakisaka and Y. Kuboki. J. Biochem. 121 (1997) 317.
J. C. Le Huec, T. Schaeverbeke, D. Clement, J. Faber and A. Le Rebeller, Biomaterials 16 (1995) 113.
M. Y. Shareef, P. F. Messer and R. Van Noort, ibid. 14 (1993) 69.
D. M. Dziedzic, I. H. Savva, D. S. Wilkinson and J. E. Davies, Mater. Res. Soc. Symp. Proc. 414 (1996) 147.
A. L. Rosa, P. V. Hatton and R. Van Noort, J. Dent. Res. 76 (1997) 1081.
M. Y. Shareef, PhD Thesis, University of Sheffield, Sheffield, UK (1991) p. 143.
A. L. Rosa, M. Y. Shareef and R. Van Noort, Braz. Oral Res. 14 (2000) 277.
C. Maniatopoulos, A. Rodriguez, D. A. Deporter and A. H. Melcher, Int. J. Oral Maxillofac. Implants 1 (1986) 36.
J. E. Davies and N. Baldan, J. Biomed. Mater. Res. 36 (1997) 429.
J. E. Davies, Anat. Rec. 245 (1996) 426.
K. A. Hing, S. M. Best, K. E. Tanner, W. Bonfiled and P. A. Revell, J. Mater. Sci.: Mater. Med. 10 (1999) 663.
A. D. Puleo and A. Nanci, Biomaterials 20 (1999) 2311.
M. Jarcho, J. F. Kay, K. I. Gumaer, R. H. Doremus and H. P. Drobeck, J. Bioeng. 1 (1977) 79.
H. W. Denissen, K. De Groot, P. C. Makkes, A. Van Den Hoof and P. J. Klopper, J. Biomed. Mater. Res. 14 (1980) 713.
I. M. Brook, G. T. Graig and D. J. Lamb, Biomaterials 12 (1991) 79.
J. D. Bruijn, C. P. A. T. Klein, K. De Groot and C. A. Blitterwijk, J. Biomed. Mater. Res. 26 (1992) 1365.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Rosa, A.L., Beloti, M.M., Oliveira, P.T. et al. Osseointegration and osseoconductivity of hydroxyapatite of different microporosities. Journal of Materials Science: Materials in Medicine 13, 1071–1075 (2002). https://doi.org/10.1023/A:1020305008042
Issue Date:
DOI: https://doi.org/10.1023/A:1020305008042