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Effects of Short-Chain Fatty Acid-Supplemented Total Parenteral Nutrition on Intestinal Pro-Inflammatory Cytokine Abundance

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Abstract

We examined the effect of short-chain fatty acid-supplemented total parenteral nutrition on proinflammatory cytokine levels in piglets. Piglets (N = 22) received either standard total parenteral nutrition or total parenteral nutrition supplemented with short-chain fatty acids. After seven days of continuous nutrient infusion, proinflammatory cytokine (TNF-α, IL-1β, IL-6) abundance in plasma, jejunal, and ileal samples and small intestinal myeloperoxidase was determined using western blotting. No differences were seen in TNF-α small intestinal abundance. IL-1β was higher in the small intestine of the short-chain fatty acid group (P < 0.05). IL-6 was higher in intestinal samples of the short-chain fatty acid group (P = 0.05), with the ileum having a greater abundance of IL-6 than the jejunum (P < 0.005). No differences in proinflammatory cytokine abundance in the plasma or tissue myeloperoxidase were seen. These results indicate short-chain fatty acids beneficially increase small intestinal abundance of IL-1β and IL-6 during total parenteral nutrition administration, while not affection systemic production of these cytokines or intestinal inflammation.

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Authors and Affiliations

  1. Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, 61801, USA

    L.A. Milo, K.A. Reardon & K.A. Tappenden

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  1. L.A. Milo
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  2. K.A. Reardon
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  3. K.A. Tappenden
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Milo, L., Reardon, K. & Tappenden, K. Effects of Short-Chain Fatty Acid-Supplemented Total Parenteral Nutrition on Intestinal Pro-Inflammatory Cytokine Abundance. Dig Dis Sci 47, 2049–2055 (2002). https://doi.org/10.1023/A:1019676929875

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