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Indirect Evidence that Drug Brain Targeting Using Polysorbate 80-Coated Polybutylcyanoacrylate Nanoparticles Is Related to Toxicity

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Abstract

Purpose. To investigate the mechanism underlying the entry of the analgesic peptide dalargin into brain using biodegradable polybutylcyanoacrylate (PBCA) nanoparticles (NP) overcoated with polysorbate 80.

Methods. The investigations were carried out with PBCA NP and with non biodegradable polystyrene (PS) NP (200 nm diameter). Dalargin adsorption was assessed by HPLC. Its entry into the CNS in mice was evaluated using the tail-flick procedure. Locomotor activity measurements were performed to compare NP toxicities. BBB permeabilization by PBCA NP was studied in vitro using a coculture of bovine brain capillary endothelial cells and rat astrocytes.

Results. Dalargin loading was 11.7 µg/mg on PBCA NP and 16.5µg/ mg on PS NP. Adding polysorbate 80 to NP led to a complete desorption. Nevertheless, dalargin associated with PBCA NP and polysorbate 80 induced a potent and prolonged analgesia, which could not be obtained using PS NP in place of PBCA NP. Locomotor activity dramatically decreased in mice dosed with PBCA NP, but not with PS NP. PBCA NP also caused occasional mortality. In vitro, PBCA NP (10 µg/ml) induced a permeabilization of the BBB model.

Conclusions. A non specific permeabilization of the BBB, probably related to the toxicity of the carrier, may account for the CNS penetration of dalargin associated with PBCA NP and polysorbate 80.

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Authors and Affiliations

  1. Laboratoire de Pharmacie Galénique et Biopharmacie UPRES EA 1223, 34, rue du Jardin des Plantes, 86000, Poitiers, France

    Jean-Christophe Olivier, Romain Chauvet, Claudine Pariat & William Couet

  2. SERLIA, INSERM U.325, Institut Pasteur, Lille, France

    Laurence Fenart

  3. Université des Sciences, Faculté Jean Perrin, Lens, France

    Roméo Cecchelli

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  1. Jean-Christophe Olivier
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  2. Laurence Fenart
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  5. Roméo Cecchelli
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Correspondence to Jean-Christophe Olivier.

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Olivier, JC., Fenart, L., Chauvet, R. et al. Indirect Evidence that Drug Brain Targeting Using Polysorbate 80-Coated Polybutylcyanoacrylate Nanoparticles Is Related to Toxicity. Pharm Res 16, 1836–1842 (1999). https://doi.org/10.1023/A:1018947208597

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