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Distribution of β-Adrenoceptor Subtypes in Gastrointestinal Tract of Nondiabetic and Diabetic BB Rats A Longitudinal Study

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Abstract

The effects of aging and diabetes on thedistribution of β-adrenoceptor subtypes in the gutwere investigated in the BB rat.[125I]Cyanopindolol binding to 10-μmsections was evaluated using film autoradiography. Cyanopindolol binding to β-,β1-, andβ2-adrenoceptors was displaced by 1μM propranolol, 50 nM ICI-89-406, and 100 nMICI-118-551, respectively. β-Adrenoceptor bindingwas highest in the circular muscle of proximal colon and lowest in thepylorus of 4- to 5-month-old rats. Aging (8- to10-month-old vs. 4- to 5-month-old rats) was associatedwith increased β-adrenoceptor binding in thepylorus and reduced binding in the proximal colon.Diabetes had a time-dependent effect on the level ofβ-adrenoceptor binding. It was increased in theantral and pyloric stomach but longer periods ofdiabetes caused a reduction in β-adrenoceptorbinding in the pylorus. Those in the intestine werereduced time-dependently and involvedβ1- or β2-adrenoceptorsor both.

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Authors
  1. O. Yu
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  2. A. Ouyang
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Yu, O., Ouyang, A. Distribution of β-Adrenoceptor Subtypes in Gastrointestinal Tract of Nondiabetic and Diabetic BB Rats A Longitudinal Study. Dig Dis Sci 42, 1146–1153 (1997). https://doi.org/10.1023/A:1018877318101

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