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Recombinant or Plasma-Derived Antisecretory Factor Inhibits Cholera Toxin-Induced Increase in Evans Blue Permeation of Rat Intestinal Capillaries

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Abstract

The effect of cholera toxin on small intestinalcapillary function, utilizing the Evans blue dye method,was analyzed. The modulatory influence of plasma-derivedor recombinant human antisecretory factor on this variable was also investigated. MaleSprague-Dawley rats were briefly anesthetized withether, and a jejunal loop was constructed that waschallenged for 90 min with phosphate-buffered saline or cholera toxin. Five minutes prior to death, therats received an intravenous injection of Evans blue.The tissue content of dye in the loop was quantitatedspectrophotometrically or demonstrated histochemically. Cholera toxin increased the recovery of Evansblue; extravasation of the dye was prominent in the topof the villi, while the crypts were spared. It issuggested that the toxin caused increased transcapillary permeation of albumin in a heterogenous fashionin the gut wall. This effect of the toxin was preventedby pretreatment with the antisecretory factor.

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Authors
  1. Stefan Lange
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  2. Dick S. Delbro
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  3. Eva Jennische
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  4. Ewa Johansson
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  5. Ivar Lonnroth
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Lange, S., Delbro, D.S., Jennische, E. et al. Recombinant or Plasma-Derived Antisecretory Factor Inhibits Cholera Toxin-Induced Increase in Evans Blue Permeation of Rat Intestinal Capillaries. Dig Dis Sci 43, 2061–2070 (1998). https://doi.org/10.1023/A:1018863315666

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