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Participation of Xanthine-Xanthine Oxidase System and Neutrophils in Development of Acute Gastric Mucosal Lesions in Rats with a Single Treatment of Compound 48/80, a Mast Cell Degranulator

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Abstract

The participation of xanthine-xanthine oxidaseand neutrophils in the development of acute gastricmucosal lesions was examined in rats injected once withcompound 48/80, a mast cell degranulator. Gastric mucosal lesions appeared 0.5 hr after compound48/80 injection and developed at 3 hr. The formation ofgastric mucosal lesions at 0.5 hr after compound 48/80injection was prevented by pretreatment with anti-neutrophil antiserum and NPC 14686, anantiinflammatory agent, but not with allopurinol, axanthine oxidase inhibitor. The development of gastricmucosal lesions at 3 hr after compound 48/80 injection was prevented by pretreatment withanti-neutrophil antiserum, NPC 14686, or allopurinol.Increases in the activities of gastric mucosal xanthineoxidase and myeloperoxidase, an index of neutrophilinfiltration, and the content of lipid peroxide occurred 0.5hr after compound 48/80 injection, and these increaseswere enhanced at 3 hr. The increases in gastric mucosalmyeloperoxidase activity and lipid peroxide content at 0.5 hr after compound 48/80injection were attenuated by pretreatment withanti-neutrophil antiserum and NPC 14686, while only theincrease in gastric mucosal xanthine oxidase activity atthe same time point was arrested by allopurinolpretreatment. The increases in gastric mucosal xanthineoxidase and myeloperoxidase activities and lipidperoxide content at 3 hr after compound 48/80 treatment were attenuated by pretreatment withanti-neutrophil antiserum, NPC 14686, or allopurinol.When compound 48/80-injected rats were treated withallopurinol at 0.5 hr after compound 48/80 injection,the progression of gastric mucosal lesions at 3 hr after theinjection was almost completely prevented withinhibition of the increases in gastric mucosal xanthineoxidase and myeloperoxidase activities and lipidperoxide content. These results indicate that in ratswith a single compound 48/80 treatment neutrophilsinfiltrated into the gastric mucosa participated in thedevelopment of acute gastric mucosal lesions and that the xanthine-xanthine oxidase system in thegastric mucosa participated in the progression ratherthan the formation of the gastric mucosallesions.

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  1. Y. Ohta
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  2. T. Kobayashi
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  3. I. Ishiguro
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Ohta, Y., Kobayashi, T. & Ishiguro, I. Participation of Xanthine-Xanthine Oxidase System and Neutrophils in Development of Acute Gastric Mucosal Lesions in Rats with a Single Treatment of Compound 48/80, a Mast Cell Degranulator. Dig Dis Sci 44, 1865–1874 (1999). https://doi.org/10.1023/A:1018803025043

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