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Coordinately regulated expression of FAT/CD36 and FACS1 in rat skeletal muscle

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Abstract

Protein-mediated fatty acid uptake and intracellular fatty acid activation are key steps in fatty acid metabolism in muscle.We have examined (a) the abundance of fatty acid translocase (FAT/CD36) mRNA (a fatty acid transporter) and long-chain acyl CoA synthetase (FACS1) mRNA in metabolically heterogeneous muscles (soleus (SOL), red (RG) and white gastrocnemius (WG)), and (b) whether FAT/CD36 and FACS1 mRNAs were coordinately upregulated in red (RTA) and white tibialis muscles (WTA) that had been chronically stimulated for varying periods of time (0.25, 1, 6 and 24 h/day) for 7 days. FAT/CD36 mRNA and FACS1 mRNA abundance were scaled with (a) the oxidative capacity of muscle (SOL > RG > WG) (p < 0.05), (b) the rates of fatty acid oxidation in red and white muscles, and (c) fatty acid uptake by sarcolemmal vesicles, derived from red and white muscles. In chronically stimulated muscles (RTA and WTA), FAT/CD36 mRNA and FACS1 mRNA were up-regulated in relation to the quantity of muscle contractile activity (p < 0.05). FAT/CD36 mRNA and FACS1 mRNA up-regulation was highly correlated (r = 0.98). The coordinated expression of FAT/CD36 and FACS is likely a functional adaptive response to facilitate a greater rate of fatty acid activation in response to a greater rate of fatty acid transport, either among different types of muscles or in muscles in which capacity for fatty acid metabolism has been enhanced.

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Authors and Affiliations

  1. Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada

    Joost J.F.P. Luiken, Xiao-Xia Han & Arend Bonen

  2. Department of Physiology, Maastricht University, Maastricht, The Netherlands

    Joost J.F.P. Luiken

  3. Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada

    D.J. Dyck

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  1. Joost J.F.P. Luiken
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  4. Arend Bonen
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Correspondence to Arend Bonen.

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Luiken, J.J., Han, XX., Dyck, D. et al. Coordinately regulated expression of FAT/CD36 and FACS1 in rat skeletal muscle. Mol Cell Biochem 223, 61–69 (2001). https://doi.org/10.1023/A:1017948726767

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