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KAP-2, a protein that binds to the H-box in a bean chalcone synthase promoter, is a novel plant transcription factor with sequence identity to the large subunit of human Ku autoantigen

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Abstract

The KAP-2 protein that binds to the H-box (CCTACC) element in the bean CHS15 chalcone synthase promoter was purified, and internal peptide sequence used to design primers leading to the cloning of KAP-2 from bean (Phaseolus vulgaris) and barrel medic (Medicago truncatula). KAP-2 shares sequence similarity to the large subunit of mammalian Ku autoantigen, a protein proposed to be involved in control of DNA recombination and transcription. KAP-2 sequences were present in genomic DNA from a range of legumes, and a related protein is found in Arabidopsis thaliana. Recombinant KAP-2 expressed in insect cells showed the same binding specificity for the CHS15 H-box as the protein purified from bean cell extracts. In vitro transcription assays confirmed that KAP-2 stimulates transcription from a promoter harboring the H-box cis element.

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Authors and Affiliations

  1. Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK , 73401, USA

    William P. Lindsay, Fiona M. McAlister, Xian-Zhi He & Richard A. Dixon

  2. Plant Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA , 92138, USA

    Qun Zhu, Wolfgang Dröge-Laser, Susie Hedrick, Peter Doerner & Chris Lamb

  3. Central Research and Development, DuPont Company, PO Box 80402, Wilmington, DE, 19880, USA

    Qun Zhu

  4. Albrecht-von-Haller-Institut, Universität Göttingen, Untere Karspüle 2, 37073, Göttingen, Germany

    Wolfgang Dröge-Laser

  5. John Innes Centre, Norwich, NR4 7UH, UK

    Chris Lamb

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  1. William P. Lindsay
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  2. Fiona M. McAlister
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Lindsay, W.P., McAlister, F.M., Zhu, Q. et al. KAP-2, a protein that binds to the H-box in a bean chalcone synthase promoter, is a novel plant transcription factor with sequence identity to the large subunit of human Ku autoantigen. Plant Mol Biol 49, 503–514 (2002). https://doi.org/10.1023/A:1015505316379

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