Skip to main content
SpringerLink
Log in

Donor Oocyte Cytoplasmic Transfer Did Not Enhance Implantation of Embryos of Women with Poor Ovarian Reserve

  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose : To determine whether donor oocyte cytoplasm transferred into the oocytes of women ≥40 years or with diminished ovarian reserve would enhance embryo quality, implantation, or pregnancy rates.

Methods : Study subjects included women ≥40 years (15) or with abnormal FSH levels (3). Healthy volunteers (18) produced oocytes for cryopreservation. Donor oocytes were thawed and cytoplasm from surviving oocytes was injected with a single sperm into the cytoplasm of recipient oocytes. Outcome measures included embryo quality scores, implantation, and pregnancy rates.

Results : Eighteen donors produced 213 oocytes for cryopreservation and 39/171 (22.8%) survived thawing. Eighteen recipients initiated 25 IVF cycles with embryo transfer in 20 cycles after cytoplasmic transfer (CT). Four cycles resulted in three biochemical losses and one aneuploid clinical loss. Embryo quality did not improve with CT compared to pre-CT IVF cycles in six recipients.

Conclusions : CT with cryopreserved donor oocyte cytoplasm did not enhance success in women with advanced reproductive age or low ovarian reserve.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Sharara FI, Scott RT Jr, Seifer DB: The detection of diminished ovarian reserve in infertile women. Am J Obstet Gynecol 1998;179:804–812

    Google Scholar 

  2. Scott RT, Opsahl MS, Leonardi MR, Neall GS, Illions EH, Navot D: Life table analysis of pregnancy rates in a general infertility population relative to ovarian reserve and patient age. Hum Reprod 1995;10:1706–1710

    Google Scholar 

  3. Battaglia DE, Goodwin P, Klein NA, Soules MR: Influence of maternal age on meiotic spindle assembly in oocytes from naturally cycling women. Hum Reprod 1996;11:2217–2222

    Google Scholar 

  4. Cohen J, Scott R, Schimmel T, Levron J, Willadsen S: Birth of infant after transfer of anucleate donor oocyte cytoplasm into recipient eggs. Lancet 1997;350:186–187

    Google Scholar 

  5. Lanzendorf SE, Mayer JF, Toner J, Oehninger S, Saffan DS, Muasher S: Pregnancy following transfer of ooplasm from cryopreserved-thawed donor oocytes into recipient oocytes. Fertil Steril 1999;71:575–577

    Google Scholar 

  6. Levron J, Willadsen S, Bertoli M, Cohen J: The development of mouse zygotes after fusion with synchronous and asynchronous cytoplasm. Hum Reprod 1996;11:1287–1292

    Google Scholar 

  7. Eppig JJ: Coordination of nuclear and cytoplasmic oocyte maturation in eutherian mammals. Reprod Fertil Dev 1996;8: 485–489

    Google Scholar 

  8. Van Blerkom J, Sinclair J, Davis P: Mitochondrial transfer between oocytes: Potential applications of mitochondrial donation and the issue of heteroplasmy.HumReprod 1998;13:2857–2868

    Google Scholar 

  9. Cohen J, Scott R, Alikani M, Schimmel T, Munne S, Levron J, Wu L, Brenner C, Warner C, Willadsen S: Ooplasmic transfer in mature human oocytes. Mol Hum Reprod 1998;4:269–280

    Google Scholar 

  10. Staessen C, Camus M, Bollen N, Devroey P, Van Steirteghem AC: The relationship between embryo quality and the occurrence of multiple pregnancies. Fertil Steril 1992;57:626–630

    Google Scholar 

  11. Martini E, Flaherty SP, Swann NJ, Matthews CD, Ramaekers FC, Geraedts JP: FISH analysis of six chromosomes in unfertilized human oocytes after polar body removal. J Assist Reprod Genet 2000;17:276–283

    Google Scholar 

  12. Lim AS, Tsakok F: Age-related decline in fertility: A link to degenerative oocytes? Fertil Steril 1997;68:265–271

    Google Scholar 

  13. Van Blerkom J, Henry G: Oocyte dysmorphism and aneuploidy in meiotically mature human oocytes after ovarian stimulation. Hum Reprod 1992;7:379–390

    Google Scholar 

  14. Wu J, Zhang L, Wang X: Maturation and apoptosis of human oocytes in vitro are age-related. Fertil Steril 2000;74: 1137–1141

    Google Scholar 

  15. Battaglia DE, Goodwin P, Klein NA, Soules MR: Influence of maternal age on meiotic spindle assembly in oocytes from naturally cycling women. Hum Reprod 1996;11: 2217–2222

    Google Scholar 

  16. Barritt JA, Brenner CA, Cohen J, Matt DW: Mitochondrial DNA rearrangements in human oocytes and embryos. Mol Hum Reprod 1999;5:927–933

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Genetics & IVF Institute, Fairfax, Virginia

    M. S. Opsahl, L. P. Thorsell, M. E. Geltinger, M. A. Iwaszko, K. L. Blauer & R. J. Sherins

Authors
  1. M. S. Opsahl
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. P. Thorsell
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. E. Geltinger
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. A. Iwaszko
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. K. L. Blauer
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. R. J. Sherins
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Opsahl, M.S., Thorsell, L.P., Geltinger, M.E. et al. Donor Oocyte Cytoplasmic Transfer Did Not Enhance Implantation of Embryos of Women with Poor Ovarian Reserve. J Assist Reprod Genet 19, 113–117 (2002). https://doi.org/10.1023/A:1014728603309

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1014728603309

Search

Navigation