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A convenient and rapid method for genetic transformation of E. coli with plasmids

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Abstract

A convenient and rapid method for the genetic transformation of Escherichia coliwith plasmids is proposed. By mixing the recipient cells and plasmid DNA and spreading them directly on selective medium plates containing Ca2+, the so-called `plate transformation' could achieve almost the same transformation efficiency as the classical transformation method with calcium. The whole protocol takes only about 2 min, its simplicity compared favorably, not only to the usual protocol, but also to all other documented modifications.

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Authors and Affiliations

  1. College of life Sciences, Wuhan University, Wuhan, 430072, PR China

    Xiangdong Chen, Peiyi Guo, Zhixiong Xie & Ping Shen

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  1. Xiangdong Chen
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  2. Peiyi Guo
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  3. Zhixiong Xie
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  4. Ping Shen
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Correspondence to Ping Shen.

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Chen, X., Guo, P., Xie, Z. et al. A convenient and rapid method for genetic transformation of E. coli with plasmids. Antonie Van Leeuwenhoek 80, 297–300 (2001). https://doi.org/10.1023/A:1013040812987

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