Abstract
Effects of three extracts (hexanes, ether, and ethanol) from tarbush (Flourensia cernua) on intake of alfalfa pellets by lambs were examined. Forty-five ewe lambs were fed one of five treatments for five days (randomized complete block, three lambs per block on each treatment). Treatments were alfalfa pellets (CON) or alfalfa pellets plus ethanol carrier (CAR), hexanes extract (HEX), ether extract (ETH), or ethanol extract (ETOH). Extracts were applied to alfalfa pellets at the same concentration as in an equivalent amount of tarbush (as fed basis) in experiment 1 and at 10-fold dilutions of that concentration in experiment 2. Treatments were isolated from tarbush leaves by using a sequential extraction with hexanes, diethyl ether, and 100% ethanol. Lambs received 640 g of alfalfa pellets (dry matter basis) each morning and intake was monitored during a 20-min interval. Lambs were maintained and fed alfalfa pellets (4.7% of body weight) as one group except during this interval. In experiment 1, mean intake by lambs during the 20-min interval was 361, 393, 204, 212, and 228 g for CON, CAR, HEX, ETH, and ETOH, respectively (SEM = 28.9). All three extracts decreased intake (P < 0.001) compared to CON or CAR. Intake did not differ among the three extracts (HEX, ETH, and ETOH) or between the two controls (CON and CAR). Mean intake did not differ among treatments in experiment 2 (468, 455, 389, 381, and 431 g for CON, CAR, HEX, ETH, and ETOH, respectively; SEM = 30.5; P = 0.187). Several compounds are probably responsible for the low palatability and differential use of tarbush typically exhibited by livestock.
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REFERENCES
ANDERSON, D. M. and HOLECHEK, J. L. 1983. Diets obtained from esophageally fistulated heifers and steers simultaneously grazing semidesert tobosa rangeland. Proc. West. Sect. Am. Soc. Anim. Sci. 34:161–164.
AOAC. 1990. Official Methods of Analysis. Association of Official Analytical Chemists, Arlington, Virginia.
AREGULLIN-GALLARDO, M. 1985. Chemical and biological significance of benzofurans and benzopyrans in the Asteraceae. PhD dissertation. University of California, Irvine, California.
BOHLMANN, F. and GRENZ, M. 1977. Ñber Inhaltsstoffe der Gattung Flourensia Chem. Ber. 110:295–300.
DOHI, H., YAMADA, A., and FUKUKAWA, T. 1996. Effects of organic solvent extracts from herbage on feeding behavior in goats. J. Chem. Ecol. 22:425–430.
DOLLAHITE, J. M. and ALLEN, T. J. 1975. The toxicity of the fruit of Flourensia cernua (tarbush) (blackbrush). Southwest. Vet. 28:113–117.
ESTELL, R. E., ANDERSON, D. M., and HAVSTAD, K. M. 1994a. Effects of organic solvents on use of tarbush by sheep. J. Chem. Ecol. 20:1137–1142.
ESTELL, R. E., FREDRICKSON, E. L., ANDERSON, D. M., MUELLER, W. F., and REMMENGA, M. D. 1994b. Relationship of tarbush leaf surface secondary chemistry to livestock herbivory. J. Range Manage. 47:424–428.
ESTELL, R. E., FREDRICKSON, E. L., and HAVSTAD, K. M. 1996. Chemical composition of Flourensia cernua at four growth stages. Grass Forage Sci. 51:434–441.
ESTELL, R. E., FREDRICKSON, E. L., ANDERSON, D. M., HAVSTAD, K. M., and REMMENGA, M. D. 1998a. Relationship of leaf surface terpene profile of tarbush with livestock herbivory. J. Chem. Ecol. 24:1–12.
ESTELL, R. E., FREDRICKSON, E. L., TELLEZ, M. R., HAVSTAD, K. M., SHUPE, W. L., ANDERSON, D. M., and REMMENGA, M. D. 1998b. Effect of volatile compounds on consumption of alfalfa pellets by sheep. J. Anim. Sci. 76:228–233.
ESTELL, R. E., FREDRICKSON, E. L., ANDERSON, D. M., HAVSTAD, K. M., and REMMENGA, M. D. 2000. Effects of individual terpenes on consumption of alfalfa pellets by sheep. J. Anim. Sci. 78:1636–1640.
FREDRICKSON, E., THILSTED, J., ESTELL, R., and HAVSTAD, K. 1994. Effects of chronic ingestion of tarbush (Flourensia cernua) on ewe lambs. Vet. Hum. Toxicol. 36:409–415.
HANKS, D. L., MCARTHUR, E. D., PLUMMER, A. P., GIUNTA, B. C., and BLAUER, A. C. 1975. Chromatographic recognition of some palatable and unpalatable subspecies of rubber rabbitbrush in and around Utah. J. Range Manage. 28:144–148.
KING, D. W., ESTELL, R. E., FREDRICKSON, E. L., HAVSTAD, K. M., WALLACE, J. D., and MURRAY, L. W. 1996. Effects of Flourensia cernua ingestion on intake, digestion, and ruminal fermentation of sheep consuming tobosa. J. Range Manage. 49:325–330.
KRUEGER, C. W., LAYCOCK, W. A., and PRICE, D. A. 1974. Relationships of taste, smell, sight and touch to forage selection. J. Range Manage. 27:258–262.
MATHEWS, F. P. 1944. The toxicity of the ripe fruit of blackbrush tarbush (Flourensia cernua) for sheep and goats. Texas Agric. Exp. Stn. Bull. No. 664.
NELSON, A. B., HERBEL, C. H., and JACKSON, H. M. 1970. Chemical composition of forage species grazed by cattle on an arid New Mexico range. New Mexico Agric. Exp. Stn. Bull. No. 561.
NOLTE, D. L., MASON, J. R., and LEWIS, S. L. 1994. Tolerance of bitter compounds by an herbivore, Cavia porcellus. J. Chem. Ecol. 20:303–308.
OH, H. K., SAKAI, T., JONES, M. B., and LONGHURST, W. M. 1967. Effect of various essential oils isolated from Douglas fir needles upon sheep and deer rumen microbial activity. Appl. Microbiol. 15:777–784.
PASS, D. M., FOLEY, W. J., and BOWDEN, B. 1998. Vertebrate herbivory on Eucalyptus-identification of specific feeding deterrents for common ringtail possums (Pseudocheirus peregrinus) by bioassay-guided fractionation of Eucalyptus ovata foliage. J. Chem. Ecol. 24: 1513–1527.
PROVENZA, F. D., BURRITT, E. A., CLAUSEN, T. P., BRYANT, J. P., REICHARDT, P. B., and DISTEL, R. A. 1990. Conditioned flavor aversion: A mechanism for goats to avoid condensed tannins in blackbrush. Am. Nat. 136:810–828.
RAO, M. M., KINGSTON, D. G. I., and SPITTLER, T. D. 1970. Flavonoids from Flourensia cernua. Phytochemistry 9:227–228.
SAS Institute. 1989. SAS/STAT User's Guide, Version 6, 4th ed., Vol. 2. SAS Institute, Inc., Cary, North Carolina.
SCHWARTZ, C. C., NAGY, J. G., and REGELIN, W. L. 1980. Juniper oil yield, terpenoid concentration, and antimicrobial effects on deer. J. Wildl. Manage. 44:107–113.
SINCLAIR, A. R. E., KREBS, C. J., SMITH, J. N. M., and BOUTIN, S. 1988. Population biology of snowshoe hares. III. Nutrition, plant secondary compounds and food limitation. J. Anim. Ecol. 57:787–806.
TELLEZ, M., ESTELL, R., FREDRICKSON, E., POWELL, J., WEDGE, D., SCHRADER, K., and KOBAISY, M. 2001. Extracts of Flourensia cernua (L): Volatile constituents and antifungal, antialgal, and antitermite bioactivities. J. Chem. Ecol. 27:2265–2277.
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Estell, R., Tellez, M., Fredrickson, E. et al. Extracts of Flourensia cernua Reduce Consumption of Alfalfa Pellets by Sheep. J Chem Ecol 27, 2275–2285 (2001). https://doi.org/10.1023/A:1012235121852
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DOI: https://doi.org/10.1023/A:1012235121852