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Migratory activity of human glioma cell lines in vitro assessed by continuous single cell observation

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Abstract

A new migration assay, the time-lapse individual cell migration assay (TIM-assay), was developed, which allows the observation of cells over 24 h under controlled conditions. Using this technique, the migratory behavior of 8 human glioblastoma cell lines in vitro was studied. Special features are simultaneous documentation of migratory parameters of individual cells, i.e., migration velocities and migration paths of individual cells. Migration velocity for cell populations of the same cell line ranged from 0 to 24 μm/h. The migration paths were examined for being directional. Two thirds of all cells showed directional migration. Migration paths were further classified according to visual judgements for being linear, oscillating or mixed. The migration index had a mean of 91%. The presented TIM-assay allows the assessment of several new parameters, that may be useful to identify subgroups of gliomas with different biological characteristics.

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Authors and Affiliations

  1. Department of Neurosurgery, Neurooncology Laboratory, Germany

    Tim Demuth, Nikolai J. Hopf, Monika Herr & Axel Perneczky

  2. Institute of Neurosurgical Pathophysiology, Germany

    Oliver Kempski

  3. Institute of Neuroradiology, University Hospital, Mainz, Germany

    Dieter Sauner

  4. Department of Neurosurgery, University Hospital, Eppendorf, Hamburg, Germany

    Alf Giese

Authors
  1. Tim Demuth
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  2. Nikolai J. Hopf
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  3. Oliver Kempski
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  4. Dieter Sauner
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  5. Monika Herr
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  6. Alf Giese
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  7. Axel Perneczky
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Demuth, T., Hopf, N.J., Kempski, O. et al. Migratory activity of human glioma cell lines in vitro assessed by continuous single cell observation. Clin Exp Metastasis 18, 589–597 (2000). https://doi.org/10.1023/A:1011927004307

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