Abstract
A mechanism of activation of protein biosynthesis in hepatocytes was proposed as effected by the conditioned medium of nonparenchymal liver cells incubated in the presence of high density lipoproteins, cortisol, and lipopolysaccharides. It was found that the increase in the biosynthesis rate was associated with the formation of the tetrahydrocortisol–apolipoprotein A-I (THC–apoA-I) complex in macrophages, which display 5α- and 5β-reductase activity and are constituents of nonparenchymal liver cell. Using the small-angle X-ray scattering technique, it was shown that the THC–apoA-I–eukaryotic DNA interaction may break hydrogen bonds between pairs of complementary nucleic bases and cause the formation of single-stranded DNA fragments capable of binding to DNA-dependent RNA polymerase. The interaction is highly cooperative and has a saturating mode, up to six enzyme molecules being bound per DNA molecule.
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REFERENCES
Robey, R.A., Jones, K.D., Tanaka, T., and Liu, T.J., J.Biol. Chem., 1984, vol. 259, pp. 7311–7316.
Sawyer, N.J., Oliver, J.T., and Troop, R.S., Steroids, 1963, vol. 2, pp. 213–227.
Schlepper-Schafer, J., Raffelsiefer, A., and Kolb-Bachofen, V., Cells of the Hepatic Sinusoid, Wisse, E., Knook, D., and Decker, K., Eds., Kupffer Cell Foundation, 1989, vol. 2, pp. 115–119.
Panin, L.E., Usynin, I.F., Trubitsyna, O.M., Khar'kovskii, A.V., Sokolova, M.V., and Ostanina, L.S., Biokhimiya, 1994, vol. 59, pp. 353–359.
Yudaev, N.A., Afinogenova, S.A., and Krekhova, M.A., Biokhimiya gormonov i gormonal'noi regulyatsii (Biochemistry of Hormones and Hormonal Regulation), Moscow: Nauka, 1976, pp. 171–227.
Panin, L.E., Vopr. Med. Khim, 1987, vol. 33, pp. 96–102.
Panin, L.E., Byull. SO RAMN, 1996, no. 2, pp. 62–69.
Panin, L.E., Byull. SO RAMN, 1998, no. 3, pp. 5–10.
Tuzikov, F.V., Zinoviev, V.V., Malygin, E.G., Ankilova, V.N., Moor, N.A., and Lavrik, O.I., FEBS Lett., 1988, vol. 232, pp. 107–110.
Tuzikov, F.V., Tuzikova, N.A., Vavilin, V.I., Zinov'ev, V.V., Malygin, E.G., Favorova, O.O., Zargarova, T.A., Sudomoina, M.A., and Kiselev, L.L., Mol. Biol. (Moscow), 1991, vol. 25, pp. 740–751.
Tuzikov, F.V., Zinoviev, V.V., Vavilin, V.I., and Malygin, E.G., Studia Biophys., 1988, vol. 125, pp. 169–172.
Tuzikov, F.V., Zinoviev, V.V., Vavilin, V.I., and Malygin, E.G., Biopolymers, 1996, vol. 38, pp. 131–139.
Tuzikov, F.V., Panin, L.E., Tuzikova, N.A., and Polyakov, L.M., Biol. Membrany, 1996, vol. 13, pp. 71–78.
Tuzikov, F.V., Tuzikova, N.A., Panin, L.E., Nikitin, Yu.P., and Krylova, I.N., Biol. Membrany, 1998, vol. 15, pp. 421–433.
Tuzikov, F.V., Tuzikova, N.A., Panin, L.E., and Nikitin,Yu.P., Vestnik RAMN, 1998, no. 3, pp. 42–47.
Stockel, P., May, R.P., Strell, I., Cejka, Z., Hoppe, W., and Heumann, H., Eur. J. Biochem., 1980, vol. 112, pp. 411–417.
Meisenberger, O., Heumann, H., and Pilz, I., FEBS Lett., 1981, vol. 123, pp. 22–24.
Heumann, H., Lederer, H., Baer, G., May, R.P., Kjems, J.K., and Crespi, H.L., J. Mol. Biol., 1988, vol. 201, pp.115–125.
Gordon, J.I., Smith, D.P., Andy, R., and Alpers, D.H., J.Biol. Chem., 1982, vol. 257, pp. 971–978.
Svergun, D.I. and Feigin, L.A., Rentgenovskoe i neitronnoe malouglovoe rasseyanie (X-ray and Neutron Small-Angle Scattering), Moscow: Nauka, 1986, pp. 50–279.
Watson, J.D., Molecular Biology of the Gene, 3rd Ed., Menlo Park, CA: Benjamin-Cummings, 1976. Translated under the title Molekulyarnaya biologiya gena, Moscow: Mir, 1978, pp. 285–288.
Panin, L.E., Tuzikov, F.V., Tuzikova, N.A., Gimautdinova, O.I., and Polyakov, L.M., Biofizika, 2000, vol. 45, pp. 611–619.
Seglen, P., Methods in Cell Biology, Prescott, D.M., Ed., New York: Academic, 1976, vol. 13, pp. 29–83.
Usynin, I.F., Novye metody nauchnykh issledovanii v klinicheskoi i eksperimental'noi meditsine (Novel Methods of Studies in Clinical and Experimental Medicine), Kaznacheev, V.P., Ed., Novosibirsk: SO AMN SSSR, 1980, pp. 96–98.
Knook, D.L., Blansjaar, N., and Sleyster, E.C., Exp. Cell Res., 1977, no. 109, pp. 317–329.
Hatch, F.T. and Lees, R.S., Adv. Lipid. Res., 1968, vol. 6, pp. 2–68.
Panin, L.E., Biushkina, M.G., and Polyakov, L.M., Byull. Eksp. Biol. Med., 1992, vol. 112, pp. 34–36.
Maniatis, T., Fritsch, E., and Sambrook, J., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, NY: Cold Spring Harbor Lab., 1982. Translated under the title Metody geneticheskoi inzhenerii. Molekulyarnoe klonirovanie, Moscow: Mir, 1984, p. 272.
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Panin, L.E., Tuzikov, F.V., Tuzikova, N.A. et al. The Effect of Tetrahydrocortisol–Apolipoprotein A-I Complex on the RNA Polymerase Interaction with Eukaryotic DNA and the Rate of Protein Biosynthesis in Hepatocytes. Russian Journal of Bioorganic Chemistry 27, 95–100 (2001). https://doi.org/10.1023/A:1011381019423
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DOI: https://doi.org/10.1023/A:1011381019423