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Disparate cleavage of poly-(ADP-ribose)-polymerase (PARP) and a synthetic tetrapeptide, DEVD, by apoptotic cells

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Abstract

In the present investigations, we have shown differential cleavage of cellular PARP and a caspase 3-selective synthetic tetrapeptide substrate, Z-DEVD-AFC or Ac-DEVD-AMC using a T lymphoblastoid cell line Jurkat, and its variant clone E6.1(J-E6). Anti-Fas antibody-mediated apoptosis resulted in DNA fragmentation and PARP cleavage in both Jurkat and J-E6 cells. However, unlike Jurkat, J-E6 cells did not cleave a synthetic tetrapeptide substrate efficiently. The failure to cleave the DEVD tetrapeptide by apoptotic J-E6 cells was not due to insufficient expression or processing of caspase 3 in J-E6 cells. Interestingly, when the J-E6 cells were transiently transfected with a cDNA encoding caspase 3, efficient cleavage of Z-DEVD-AFC was achieved. The observations that apoptotic J-E6 cells barely cleaved a synthetic DEVD tetrapeptide, but efficiently cleaved endogenous PARP, potentially at the most preferred DEVD site, suggest that active caspases may have disparate characteristics to recognize substrates presented in different context.

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References

  1. Raff M. Neural development: Mysterious no more? Science 1996; 274: 1063.

    Google Scholar 

  2. Jacobson MD, Weil M, Raff MC. Programmed cell death in animal development. Cell 1997; 88: 347-354.

    Google Scholar 

  3. Boise LH, Noel PJ, Thompson CB. CD28 apoptosis. Curr Opin Immunol 1995; 7: 620-625.

    Google Scholar 

  4. Ishizaki Y, Cheng L, Mudge AW, Raff MC. Programmed cell death by default in embryonic cells, fibroblasts, and cancer cells. Mol Biol Cell 1995; 6: 1443-1458.

    Google Scholar 

  5. Krammer PH, Behrmann I, Daniel P, Dhein J, Debatin KM. Regulation of apoptosis in the immune system. Curr Opin Immunol 1994; 6: 279-289.

    Google Scholar 

  6. Nagata S, Suda T. Fas Fas ligand: lpr and gld mutations. Immunol Today 1995; 16: 39-43.

    Google Scholar 

  7. Nagata S. Fas-induced apoptosis diseases caused by its abnormality. Genes to Cells 1996; 1: 873-879.

    Google Scholar 

  8. Nagata S. Human autoimmune lymphoproliferative syndrome, a defect in the apoptosis-inducing Fas receptor: A lesson from the mouse model. J Hum Genetics 1998; 43: 2-8.

    Google Scholar 

  9. Rudin CM, Thompson CB. Apoptosis and disease: Regulation and clinical relevance of programmed cell death. Ann Rev Med 1997; 48: 267-281.

    Google Scholar 

  10. Kerr JF, Gobe GC, Winterford CM, Harmon BV. Anatomical methods in cell death. Meth Cell Biol 1995; 46: 1-27.

    Google Scholar 

  11. Chinnaiyan AM, Dixit VM. The cell-death machine. Curr Biol 1996; 6: 555-562.

    Google Scholar 

  12. Dragovich T, Rudin CM, Thompson CB. Signal transduction pathways that regulate cell survival and cell death. Oncogene 1998; 17: 3207-3213.

    Google Scholar 

  13. Dhein J, Daniel PT, Trauth BC, Oehm A, Moller P, Krammer PH. Induction of apoptosis by monoclonal antibody anti-APO-1 class switch variants is dependent on cross-linking of APO-1 cell surface antigens. J Immunol 1992; 149: 3166-3173.

    Google Scholar 

  14. Ashkenazi A, Dixit VM. Death receptors: Signaling and modulation. Science 1998; 281: 1305-1308.

    Google Scholar 

  15. Nicholson DW, Thornberry NA. Caspases: Killer proteases. Trends Biochem Sci 1997; 22: 299-306.

    Google Scholar 

  16. Enari M, Hug H, Nagata S. Involvement of an ICE-like protease in Fas-mediated apoptosis. Nature 1995; 375: 78-81.

    Google Scholar 

  17. Alnemri ES. Mammalian cell death proteases: A family of highly conserved aspartate specific cysteine proteases. J Cell Biochem 1997; 64: 33-42.

    Google Scholar 

  18. Thornberry NA, Lazebnik Y. Caspases: Enemies within. Science 1998; 281: 1312-1316.

    Google Scholar 

  19. Thornberry NA, Rano TA, Peterson EP, et al. A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis. J Biol Chem 1997; 272: 17907-17911.

    Google Scholar 

  20. Salvesen GS, Dixit VM. Caspases: Intracellular signaling by proteolysis. Cell 1997; 91: 443-446.

    Google Scholar 

  21. Chinnaiyan AM, Orth K, O'Rourke K, Duan H, Poirier GG, Dixit VM. Molecular ordering of the cell death pathway. Bcl-2 and Bcl-xL function upstream of the CED-3-like apoptotic proteases. J Biol Chem 1996; 271: 4573-4576.

    Google Scholar 

  22. Orth K, O'Rourke K, Salvesen GS, Dixit VM. Molecular ordering of apoptotic mammalian CED-3/ICE-like proteases. J Biol Chem 1996; 271: 20977-20980.

    Google Scholar 

  23. Enari M, Talanian RV, Wong WW, Nagata S. Sequential activation of ICE-like and CPP32-like proteases during Fas-mediated apoptosis. Nature 1996; 380: 723-726.

    Google Scholar 

  24. Casciola-Rosen L, Nicholson DW, Chong T, et al. Apopain/CPP32 cleaves proteins that are essential for cellular repair: A fundamental principle of apoptotic death. J Exp Med 1996; 183: 1957-1964.

    Google Scholar 

  25. Casciola-Rosen LA, Miller DK, Anhalt GJ, Rosen A. Specific cleavage of the 70-kDa protein component of the U1 small nuclear ribonucleoprotein is a characteristic biochemical feature of apoptotic cell death. J Biol Chem 1994; 269: 30757-30760.

    Google Scholar 

  26. Lazebnik YA, Kaufmann SH, Desnoyers S, Poirier GG, Earnshaw WC. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature 1994; 371: 346-347.

    Google Scholar 

  27. Kaufmann SH, Desnoyers S, OttavianoY, Davidson NE, Poirier GG. Specific proteolytic cleavage of poly(ADP-ribose) polymerase: An early marker of chemotherapy-induced apoptosis. Cancer Res 1993; 53: 3976-3985.

    Google Scholar 

  28. D'Amours D, Germain M, Orth K, Dixit VM, Poirier GG. Proteolysis of poly(ADP-ribose) polymerase by caspase 3: Kinetics of cleavage of mono(ADP-ribosyl)ated and DNA-bound substrates. Radiation Res 1998; 150: 3-10.

    Google Scholar 

  29. Nicholson DW, Ali A, Thornberry NA, et al. Identification inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature 1995; 376: 37-43.

    Google Scholar 

  30. Schlegel J, Peters I, Orrenius S, et al. CPP32/apopain is a key interleukin 1 beta converting enzyme-like protease involved in Fas-mediated apoptosis. J Biol Chem 1996; 271: 1841-1844.

    Google Scholar 

  31. Duriez PJ, Shah GM. Cleavage of poly(ADP-ribose) polymerase: A sensitive parameter to study cell death. Biochem Cell Biol 1997; 75: 337-349.

    Google Scholar 

  32. Kircheis R, Kichler A, Wallner G, et al. Coupling of cellbinding ligands to polyethylenimine for targeted gene delivery. Gene Ther 1997; 4: 409-418.

    Google Scholar 

  33. Chow SC, Weis M, Kass GE, Holmstrom TH, Eriksson JE, Orrenius S. Involvement of multiple proteases during Fasmediated apoptosis in T lymphocytes. FEBS Lett 1995; 364: 134-138.

    Google Scholar 

  34. Faleiro L, Kobayashi R, Fearnhead H, Lazebnik Y. Multiple species of CPP32 and Mch2 are the major active caspases present in apoptotic cells. EMBO J 1997; 16: 2271-2281.

    Google Scholar 

  35. Kuida K, Zheng TS, Na S, et al. Decreased apoptosis in the brain and premature lethality in CPP32-deficient mice. Nature 1996; 384: 368-372.

    Google Scholar 

  36. Martins LM, Kottke T, Mesner PW, et al. Activation of multiple interleukin-1beta converting enzyme homologues in cytosol and nuclei of HL-60 cells during etoposide-induced apoptosis. J Biol Chem 1997; 272: 7421-7430.

    Google Scholar 

  37. Jones RA, Johnson VL, Hinton RH, Poirier GG, Chow SC, Kass GE. Liver poly(ADP-ribose)polymerase is resistant to cleavage by caspases. Biochem Biophys Res Comm 1999; 256: 436-441.

    Google Scholar 

  38. Fernandes-Alnemri T, Armstrong RC, Krebs J, et al. In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains. Proc Natl Acad Sci USA 1996; 93: 7464-7469.

    Google Scholar 

  39. Han Z, Hendrickson EA, Bremner TA, Wyche JH. Asequential two-step mechanism for the production of the mature p17:p12 form of caspase-3 in vitro. J Biol Chem 1997; 272: 13432-13436.

    Google Scholar 

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Authors and Affiliations

  1. Boehringer Ingelheim Pharmaceuticals, Inc., 900 Ridgebury Road, Ridgefield, CT, 06877, USA

    K. Yu, C. A. Kennedy, M. M. O'Neill & R. W. Barton

  2. Boehringer Ingelheim Pharmaceuticals, Inc., 900 Ridgebury Road, Ridgefield, CT, 06877, USA

    R. J. Tatake

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  1. K. Yu
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  2. C. A. Kennedy
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  3. M. M. O'Neill
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  5. R. J. Tatake
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Yu, K., Kennedy, C.A., O'Neill, M.M. et al. Disparate cleavage of poly-(ADP-ribose)-polymerase (PARP) and a synthetic tetrapeptide, DEVD, by apoptotic cells. Apoptosis 6, 151–160 (2001). https://doi.org/10.1023/A:1011375024832

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