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Multiple Forms of Phosphatase from Human Brain: Isolation and Partial Characterization of Affi-Gel Blue Nonbinding Phosphatase Activities

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Abstract

Phosphatases extracted from a human brain were resolved into two main groups, namely affi-gel blue-binding phosphatases and affi-gel blue-nonbinding phosphatases. Affi-gel blue binding phosphatases were further separated into four different phosphatase activities, designated P1-P4, and described previously (1). In the present study we describe the affi-gel blue-nonbinding phosphatases which were separated into seven different phosphatase activities, designated P5-P11 by poly-(L-lysine)-agarose and aminohexyl Sepharose 4B chromatographies. These seven phosphatase activities were active toward nonprotein phosphoester. P7-P11 and to some extent P5 could also dephosphorylate a phosphoprotein. They displayed different enzyme kinetics. On the basis of activity peak, the apparent molecular mass as estimated by Sephadex G-200 column chromatography for P5 was 49 kDa; P6, 32 kDa; P7, 150 kDa; P8, 250 kDa; P9, 165 kDa; P10, 90 kDa and P11, 165 kDa. Immunoblot analysis indicated that P8-P11 may belong to PP2B family, whereas P7 may associate with PP2A. The phosphatases P7-P11 were found to be effective in the dephosphorylation of Alzheimer's disease abnormally hyperphosphorylated tau. The resulting dephosphorylated tau regained its activity in promoting the microtubule assembly, suggesting that P7-P11 might regulate the phosphorylation of tau protein in the brain.

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Authors and Affiliations

  1. Department of Neurochemistry, New York State Institute for Basic Research in Developmental Disabilities, Staten Island, New York, 10314

    Li Yao Cheng, Jian-Zhi Wang, Cheng-Xin Gong, Jin-Jing Pei, Tanweer Zaidi, Inge Grundke-Iqbal & Khalid Iqbal

  2. Department of Biochemistry, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore, 119260

    Li Yao Cheng

  3. Department of Pathophysiology, Tongji Medical College, HUST, Wuhan, 430030, P.R. China

    Jian-Zhi Wang

  4. Department of Geriatric Medicine, Karolinska Institutet B56-K, S-141 86, Huddinge, Sweden

    Jin-Jing Pei

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Cheng, L.Y., Wang, JZ., Gong, CX. et al. Multiple Forms of Phosphatase from Human Brain: Isolation and Partial Characterization of Affi-Gel Blue Nonbinding Phosphatase Activities. Neurochem Res 26, 425–438 (2001). https://doi.org/10.1023/A:1010963401453

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