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Effect of Different Concentrations of Recombinant Leukemia Inhibitory Factor on Different Development Stage of Mouse Embryo In Vitro

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Abstract

Purpose: To assess the influence of different concentrationsof recombinant human leukemia inhibitory factor (LIF) onthe in vitro development of mouse embryos.

Methods: The 2- to 4-cell embryos of CB6F1 mice werecultured in the human tubal fluid (HTF) media containingdifferent concentrations of LIF. Mouse embryos were dividedinto seven groups: (1) HTF; (2) 1500 IU/ml LIF; (3) 1000IU/ml LIF; (4) 750 IU/ml LIF; (5) 500 IU/ml LIF; (6) 250IU/ml LIF; (7) 125 IU/ml LIF. The embryonic numbers ofdifferent stages including 5–8 cell, 9–16 cell, morula, blastocyst,and hatching blastocyst were recorded.

Results: The percentage of early embryo stage (2-cellembryos to 6- to 16-cell stages) in all groups were nonsignificantlydifferent. There were higher formation rates of preimplantationembryos (morula to hatching blastocyst) ingroups 2, 3, 4, and 5 than in groups 1, 6 and 7.

Conclusions: LIF has positive effects on preimplantationembryo development and has nonsignificant influence onthe early embryo development. The lowest concentration ofLIF which could provide the optimal embryo developmentis 500 IU/ml.

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REFERENCES

  1. Tsai HD, Chang CC, Hsieh YY, Lo HY, Hsu LW, Chang SC: Recombinant human leukemia inhibitory factor enhances the development of preimplantation mouse embryo in vitro. Fertil Steril 1999;71:722–725

    Google Scholar 

  2. Stewart CL, Kaspar P, Brunet LJ, Bhatt H, Gadi I, Kontgen F, et al.: Blastocyst implantation depends on maternal expression of leukemia inhibitory factor. Nature 1992;359:76–79

    Google Scholar 

  3. Michell MH, Swanson RJ, Hodgen GD, Oehninger S: Enhancement of in vitro murine embryo development by recombinant leukemia inhibitory factor. J Soc Gynecol Invest 1994;1:215–219

    Google Scholar 

  4. Fry RC, Batt PA, Fairclough RJ, Parr RA: Human leukemia inhibitory factor improves the viability of ovine embryos. Biol Reprod 1992;46:470–474

    Google Scholar 

  5. Kurzrock R, Estrov Z, Wetzler M, Gutterman JU, Talpaz M: LIF: Not just a leukemia inhibitory factor. Endocrine Rev 1991;12:208–217

    Google Scholar 

  6. Stewart CL: Leukemia inhibitory factor and the regulation of preimplantation development of the mammalian embryo. Mol Reprod Dev 1994;39:233–238

    Google Scholar 

  7. Lavranos TC, Rathjen PD, Seamark RF: Trophic effects of myeloid leukaemia inhibitory factor (LIF) on mouse embryos. J Reprod Fertil 1995;105:331–338

    Google Scholar 

  8. Eckert J, Tao T, Niemann H: Ratio of inner cell mass and trophoblastic cells in blastocysts derived from porcine 4–and 8–cell embryos and isolated blastomeres cultured in vitro in the presence or absence of protein and human leukemia inhibitory factor. Biol Reprod 1997;57:552–560

    Google Scholar 

  9. Wiemer KE, Huffman DI, Masxon WS, Eager S, Muhlberger B, Fiore I, Cuervo M: Embryonic morphology and rate of implantation of juman embryos following coculture on bovine oviductal epithelial cells. Hum Reprod 1993;8:97–101

    Google Scholar 

  10. Wiemer KE, Cohen J, Wilker SR, Malter HE, Wright G, Godke RA: Coculture of human zygotes on fetal bovine uterine fibroblasts:embryonic morphology and implnntation. Fertil Steril 1989;52:503–508

    Google Scholar 

  11. Menezo YJR, Guerin JF, Czyba JC: Improvement of juman early embryo development in vitro by coculture on monolayers of vero cells. Biol Reprod 1990;42:301–306

    Google Scholar 

  12. Watson AH, Hogan A, Hahnel A, Wiemer KE, Schultz GA: Expression of growth factor ligand and receptor genes in the preimplantation bovine embryo. Mol Reprod Dev 1992;31:87–95

    Google Scholar 

  13. Stewart CL, Kaspar P, Brunet LJ, Bhatt H, Gadi I, Kontgen F, et al.: Blastocyst implantation depends on maternal expression of leukemia inhibitory factor. Nature 1992;359:76–79

    Google Scholar 

  14. Charnok-Jones DS, Sharkey AM, Fenwick P, Smith SK: Leukemia inhibitory factor mRNA concentration peaks in human endometrium at the time of implantation and the blastocyst contains mRNA for the receptor at the time. J Reprod Fertil 1994;101:421–426

    Google Scholar 

  15. Dunglison GF, Barlow DH, Sargent IL: Leukaemia inhibitory factor significantly enhances the blastocyst formation rates of human embryos cultured in serum-free medium. Hum Reprod 1996;11:191–196

    Google Scholar 

  16. Jurisicova A, Ben-Chetrit A, Varmuza SL, Casper RF: Recombinant human leukemia inhibitory factor does not enhance in vitro human blastocyst formation. Fertil Steril 1995;64:999–1002

    Google Scholar 

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, China Medical College Hospital, Taichung, Taiwan

    Horng-Der Tsai, Chi-Chen Chang, Yao-Yuan Hsieh & Hui-Yu Lo

  2. Department of Obstetrics and Gynecology, China Medical College Hospital, No.2 Yuh-Der Road, Taichung, Taiwan

    Chi-Chen Chang

  3. Department of Medical Research, China Medical College, Taichung, Taiwan

    Li-Wei Hsu & Su-Chen Chang

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  1. Horng-Der Tsai
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  2. Chi-Chen Chang
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  3. Yao-Yuan Hsieh
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  4. Li-Wei Hsu
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  5. Su-Chen Chang
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  6. Hui-Yu Lo
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Tsai, HD., Chang, CC., Hsieh, YY. et al. Effect of Different Concentrations of Recombinant Leukemia Inhibitory Factor on Different Development Stage of Mouse Embryo In Vitro. J Assist Reprod Genet 17, 352–355 (2000). https://doi.org/10.1023/A:1009413329977

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