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Isolation and characterization of Metolachlor-resistant mutants of Saccharomyces cerevisiae

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Abstract

The herbicide Metolachlor (α-chloroacetamide group) inhibits the growth of Saccharomyces cerevisiae on complete, minimal, and non-fermentative media. Spontaneous and induced resistant mutants showed monogenic segregation patterns. Among the resistant clones, 70% were recessives, 16.4% were partially dominants and 13.4% were dominants. The spontaneous partially dominant mutation Mtc1 was mapped on linkage group XV at 33.3 cM from ade2 and 31.7 cM from his3, in a region that is characterized by the presence of several resistant genes. The recessive mutation mtc2 was located on chromosome IV. Although all the mutants had the ability to grow in the presence of the herbicide, they remained affected in their respiration efficiency, indicating two different mechanism of action of Metholachor on yeast cells.

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Authors and Affiliations

  1. Instituto de Biotecnologia, Universidade de Caxias do Sul, CP 1352, Caxias do Sul, CEP 95001-970, Rio Grande do Sul, Brazil

    S. Echeverrigaray

  2. Departamento de Genética, ESALQ, Universidade de São Paulo, CP 83, Piracicaba, CEP 13400-260, São Paulo, Brazil

    L.H. Gomes & F.C.A. Tavares

Authors
  1. S. Echeverrigaray
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  2. L.H. Gomes
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  3. F.C.A. Tavares
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Echeverrigaray, S., Gomes, L. & Tavares, F. Isolation and characterization of Metolachlor-resistant mutants of Saccharomyces cerevisiae. World Journal of Microbiology and Biotechnology 15, 679–681 (1999). https://doi.org/10.1023/A:1008943914292

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  • DOI: https://doi.org/10.1023/A:1008943914292

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