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Dynamics of the carbohydrate chains attached to the Fc portion of immunoglobulin G as studied by NMR spectroscopy assisted by selective 13C labeling of the glycans

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Abstract

A systematic method for 13C labeling of the glycan of immunoglobulin G for NMR study has been developed. A mouse immunoglobulin of subclass IgG2b has been used for the experiment. On the basis of chemical shift and linewidth data, it has been concluded that (1) the mobility of the carbohydrate chain in IgG2b is comparable to that of the backbone polypeptide chain with the exception of the galactose residue at the nonreducing end of the Manα1–3 branch, which is extremely mobile and (2) agalactosylation does not induce any significant change in the mobility. The results obtained indicate that even in the agalactosyl form the glycans are buried in the protein. Biological significance of the NMR results obtained is also briefly discussed.

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References

  • Damm, J.B.L., Kamerling, J.P., van Dedem, G.W.K. and Vliegenthart, J.F.G. (1987) Glycoconjugate J., 4, 129–144.

    Google Scholar 

  • de Beer, T., van Zuylen, C.W.E.M., Hård, K., Boelens, R., Kaptein, R., Kamerling, J.P. and Vliegenthart, J.F.G. (1994) FEBS Lett., 348, 1–6.

    Google Scholar 

  • de Beer, T., van Zuylen, C.W.E.M., Leeflang, B.R., Hård, K., Boelens, R., Kaptein, R., Kamerling, J.P. and Vliegenthart, J.F.G. (1996) Eur. J. Biochem., 241, 229–242.

    Google Scholar 

  • Deisenhofer, J. (1981) Biochemistry, 20, 2361–2370.

    Google Scholar 

  • Fujii, S., Nishiura, T., Nishikawa, A., Miura, R. and Taniguchi, N. (1990) J. Biol. Chem., 265, 6009–6018.

    Google Scholar 

  • Gilhespy-Muskett, A.M., Partridge, J., Jefferis, R. and Homans, S.W. (1994) Glycobiology, 4, 485–489.

    Google Scholar 

  • Harris, L.J., Larson, S.B., Hasel, K.W. and McPherson, A. (1997) Biochemistry, 36, 1581–1597.

    Google Scholar 

  • Homans, S.W. (1994) in Molecular Glycobiology, (Fukuda, M. and Hindsgaulf, O., Eds), Oxford University Press, New York, NY, pp 230–257.

    Google Scholar 

  • Ichikawa, Y., Lin, Y.-C., Dumas, D.P., Shen, G.-J., Garcia-Junceda, E., Williams, M.A., Bayer, R., Ketcham, C., Walker, L.E., Paulson, J.C. and Wong, C.-H. (1992) J. Am. Chem. Soc., 114, 9283–9298.

    Google Scholar 

  • Kiyohara, T., Terao, T., Shioiri-Nakano, K. and Osawa, T. (1976) J. Biochem. (Tokyo), 80, 9–17.

    Google Scholar 

  • Leatherbarrow, R.J., Rademacher, T.W., Dwek, R.A., Woof, J.M., Clark, A., Burton, D.R., Richardson, N. and Feinstein, A. (1985) Mol. Immunol., 22, 407–415.

    Google Scholar 

  • Lu, J. and van Halbeek, H. (1996) Carbohydr. Res., 296, 1–21.

    Google Scholar 

  • Malhotra, R., Wormald, M.R., Rudd, P.M., Fischer, P.B., Dwek, R.A. and Sim, R.B. (1995) Nat. Med., 1, 237–243.

    Google Scholar 

  • Mizuochi, T., Hamako, J. and Titani, K. (1987) Arch. Biochem. Biophys., 257, 387–394.

    Google Scholar 

  • Mizuochi, T., Hamako, J., Nose, M. and Titani, K. (1990) J. Immunol., 145, 1794–1798.

    Google Scholar 

  • Nezlin, R. (1990) Adv. Immunol., 48, 1–40.

    Google Scholar 

  • Nose, M. and Wigzell, H. (1983) Proc. Natl. Acad. Sci. USA, 80, 6632–6636.

    Google Scholar 

  • Parekh, R.B., Dwek, R.A., Sutton, B.J., Fernandes, D.L., Leung, A., Stanworth, D. and Rademacher, T.W. (1985) Nature (London), 316, 452–457.

    Google Scholar 

  • Rademacher, T.W., Williams, P. and Dwek, R.A. (1994) Proc. Natl. Acad. Sci. USA, 91, 6123–6127.

    Google Scholar 

  • Rosen, P., Pecht, I. and Cohen, J.S. (1979) Mol. Immunol., 16, 435–436.

    Google Scholar 

  • Rothman, R.J., Warren, L., Vliegenthart, J.F.G. and Härd, K.J. (1989) Biochemistry, 28b, 1377–1384.

    Google Scholar 

  • Sawada, J., Terao, T., Itoh, S., Maeda, M., Tsuji, A., Hosoda, H. and Nambara, T. (1987) J. Steroid Biochem., 28, 405–410.

    Google Scholar 

  • Sutton, B.J. and Phillips, D.C. (1983) Biochem. Soc. Trans., 11, 130–132.

    Google Scholar 

  • Takahashi, N., Ishii, I., Ishihara, H., Mori, M., Tejima, S., Jefferis, R., Endo, S. and Arata, Y. (1987) Biochemistry, 26, 1137–1144.

    Google Scholar 

  • Tao, M.-H. and Morrison, S.L. (1989) J. Immunol., 143, 2595–2601.

    Google Scholar 

  • Tsuchiya, N., Endo, T., Matsuta, K., Yoshinoya, S., Aikawa, T., Kosuge, E., Takeuchi, F., Miyamoto, T. and Kobata, A. (1989) J. Rheumatol., 16, 285–290.

    Google Scholar 

  • Vliegenthart, J.F.G., Dorland, L. and van Halbeek, H. (1983) Adv. Carbohydr. Chem. Biochem., 41, 209–374.

    Google Scholar 

  • Walker, T.E. and Barker, R. (1978) Carbohydr. Res., 64, 266–270.

    Google Scholar 

  • Weller, C.T., Lustbader, J., Seshadri, K., Brown, J.M., Chadwick, C.A., Kolthoff, C.E., Ramnarain, S., Pollak, S., Canfield, R. and Homans, S.W. (1996) Biochemistry, 35, 8815–8823.

    Google Scholar 

  • Wormald, M.R., Rudd, P.M., Harvey, D.J., Chang, S.-C., Scragg, I.G. and Dwek, R.A. (1997) Biochemistry, 36, 1370–1380.

    Google Scholar 

  • Wyss, D.F., Choi, J.S., Li, J., Knoppers, M.H., Willis, K.J., Arulanandam, A.R.N., Smolyar, A., Reinherz, E.L. and Wagner, G. (1995a) Science, 269, 1273–1278.

    Google Scholar 

  • Wyss, D.F., Choi, J.S. and Wagner, G. (1995b) Biochemistry, 34, 1622–1634.

    Google Scholar 

  • Yabe, N., Matsuya, Y., Yamane, I. and Takada, M. (1986) In Vitro Cell. & Dev. Biol., 22, 363–368.

    Google Scholar 

  • Yamamoto, S., Hase, S., Fukuda, S., Sano, O. and Ikenaka, T. (1989) J. Biochem. (Tokyo), 105, 547–555.

    Google Scholar 

  • Youings, A., Chang, S.-C., Dwek, R.A. and Scragg, I.G. (1996) Biochem. J., 314, 621–630.

    Google Scholar 

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Authors and Affiliations

  1. Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan

    Yoshiki Yamaguchi, Koichi Kato, Mitsuru Shindo, Shin Aoki, Kumiko Furusho, Kenji Koga & Ichio Shimada

  2. The GlycoLab, Central Research Institute, Nakano Vinegar Co. Ltd., 2-6 Nakamura-cho, Handa City, 475-0873, Japan

    Noriko Takahashi

  3. Water Research Institute, Sengen 2-1-6, Tsukuba, Ibaraki, 305-0047, Japan

    Yoji Arata

Authors
  1. Yoshiki Yamaguchi
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  2. Koichi Kato
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  3. Mitsuru Shindo
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  4. Shin Aoki
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  5. Kumiko Furusho
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  6. Kenji Koga
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  7. Noriko Takahashi
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  8. Yoji Arata
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  9. Ichio Shimada
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Yamaguchi, Y., Kato, K., Shindo, M. et al. Dynamics of the carbohydrate chains attached to the Fc portion of immunoglobulin G as studied by NMR spectroscopy assisted by selective 13C labeling of the glycans. J Biomol NMR 12, 385–394 (1998). https://doi.org/10.1023/A:1008392229694

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  • DOI: https://doi.org/10.1023/A:1008392229694

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