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Solution structure of the recombinant human oncoprotein p13MTCP1

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Abstract

The human oncoprotein p13MTCP1 is coded by the MTCP1 gene, a gene involved in chromosomal translocations associated with T-cell prolymphocytic leukemia, a rare form of human leukemia with a mature T-cell phenotype. The primary sequence of p13MTCP1 is highly and only homologous to that of p14TCL1, a product coded by the gene TCL1 which is also involved in T-cell prolymphocytic leukemia. These two proteins probably represent the first members of a new family of oncogenic proteins. We present the three-dimensional solution structure of the recombinant p13MTCP1 determined by homonuclear proton two-dimensional NMR methods at 600 MHz. After proton resonance assignments, a total of 1253 distance restraints and 64 dihedral restraints were collected. The solution structure of p13MTCP1 is presented as a set of 20 DYANA structures. The rmsd values with respect to the mean structure for the backbone and all heavy atoms for the conformer family are 1.07 ± 0.19 and 1.71 ± 0.17 Å, when the structured core of the protein (residues 11–103) is considered. The solution structure of p13MTCP1 consists of an orthogonal β-barrel, composed of eight antiparallel β-strands which present an original arrangement. The two β-pleated loops which emerge from this barrel might constitute the interaction surface with a potential molecular partner.

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Authors and Affiliations

  1. Centre de Biochimie Structurale, CNRS-UMR 9955, INSERM-U414, Université de Montpellier I, Faculté de Pharmacie, 15 Avenue Charles Flahault, F-34060, Montpellier Cedex 1, France

    Yin-Shan Yang, Laurent Guignard, André Padilla, François Hoh, Marie-Paule Strub & Christian Roumestand

  2. Unité INSERM U462, Hôpital Saint-Louis, F-75475, Paris, France

    Marc-Henri Stern & Jean-Marc Lhoste

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  1. Yin-Shan Yang
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  2. Laurent Guignard
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  3. André Padilla
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Yang, YS., Guignard, L., Padilla, A. et al. Solution structure of the recombinant human oncoprotein p13MTCP1 . J Biomol NMR 11, 337–354 (1998). https://doi.org/10.1023/A:1008279616063

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